Western blot abreview for Anti-Smad3 antibody [EP568Y]

Good
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+0.1% Tween20
Dilution
1/5000

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 55 kDa
Exposure
15 minute(s) and 0 second(s)

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Submitted Oct 24 2014

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