Western blot abreview for Anti-Smad3 antibody [EP568Y]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Saos)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Saos
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 21°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/5000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 55 kDa

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Submitted Apr 21 2015

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