Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (femur)
Specification
femur
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrated buffer, pH6.0
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1%BSA
Secondary antibody
Name
Non-Abcam antibody was used: EnVision plus
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Osteoblasts and osteocytes were stained by ab40759.It seemed that nucleus was stained much strongly than cytoplasm.
Abcam user community
Verified customer
Submitted Aug 23 2012