Application
Western blot
Sample
Coturnix japonica Tissue lysate - whole (yolk sac membrane from embryonic day 3 embryos)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Loading amount
50 µg
Specification
yolk sac membrane from embryonic day 3 embryos
Blocking step
Gelatin buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST solution
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
No negative controls
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 65 kDa
Positive control
No positive controls
Additional data
Additional Notes
I'm not sure about the secondary antibody is monoclonal or polyclonal.
Miss. HAN-JEN LIN
Verified customer
Submitted Apr 17 2018