Application
ChIP
Sample
Human Cell lysate - whole cell (MDA-MB-231 Cells treated with 2 ng/µl TGF-beta1)
Negative control
Polyclonal anti-HA antibody (for detection of non-specific binding). The graph is shown as a "fold enrichment" relative to the average detected HA binding.
Specification
MDA-MB-231 Cells treated with 2 ng/µl TGF-beta1
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Other product details
Incubation time
0 minute(s)
Dilution
1/500
Additional data
Additional Notes
The ChIP protocol employed by this Abreviewer is published as a link on the datasheet;
http://www.abcam.com/index.html?pageconfig=protocols&pid=789&intAbID=0&mode=prot
Abcam user community
Verified customer
Submitted Feb 14 2007