Overview

  • Product name

  • Description

    Rabbit polyclonal to SMAD5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Recombinant fragment within Zebrafish SMAD5 aa 2-229. The exact sequence is proprietary.
    Database link: Q9W7E7

  • Positive control

    • Zebrafish brain, heart, liver and muscle tissue extracts; zebrafish tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab211141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.

Target

  • Function

    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications

    Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • Information by UniProt
  • Database links

    • Alternative names

      • DKFZp781C1895 antibody
      • DKFZp781O1323 antibody
      • Dwfc antibody
      • hSmad5 antibody
      • JV5 1 antibody
      • JV5-1 antibody
      • MAD homolog 5 antibody
      • MAD, mothers against decapentaplegic homolog 5 antibody
      • MADH 5 antibody
      • MADH5 antibody
      • Mothers against decapentaplegic homolog 5 antibody
      • mothers against decapentaplegic, drosophila, homolog of, 5 antibody
      • Mothers against DPP homolog 5 antibody
      • MusMLP antibody
      • SMA and MAD related protein 5 antibody
      • SMAD 5 antibody
      • SMAD family member 5 antibody
      • SMAD, mothers against DPP homolog 5 antibody
      • Smad5 antibody
      • SMAD5_HUMAN antibody
      see all

    Images

    • All lanes : Anti-SMAD5 antibody (ab211141) at 1/1000 dilution

      Lane 1 : zebrafish brain tissue extract
      Lane 2 : zebrafish heart tissue extract
      Lane 3 : zebrafish liver tissue extract
      Lane 4 : zebrafish muscle tissue extract

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 52 kDa



      10% SDS-PAGE

    • Immunohistochemical analysis of paraffin-embedded zebrafish tissue labeling SMAD5 with ab211141 at 1/300 dilution.

    References

    ab211141 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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