Key features and details
- Rabbit polyclonal to SMAD5
- Suitable for: IHC-P, WB
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-SMAD5 antibody
See all SMAD5 primary antibodies
DescriptionRabbit polyclonal to SMAD5
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Zebrafish
Recombinant fragment within Zebrafish SMAD5 aa 2-229. The exact sequence is proprietary.
Database link: Q9W7E7
- Zebrafish brain, heart, liver and muscle tissue extracts; zebrafish tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 52 kDa.|
FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Sequence similaritiesBelongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
modificationsPhosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
- Information by UniProt
- DKFZp781C1895 antibody
- DKFZp781O1323 antibody
- Dwfc antibody
All lanes : Anti-SMAD5 antibody (ab211141) at 1/1000 dilution
Lane 1 : zebrafish brain tissue extract
Lane 2 : zebrafish heart tissue extract
Lane 3 : zebrafish liver tissue extract
Lane 4 : zebrafish muscle tissue extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
Immunohistochemical analysis of paraffin-embedded zebrafish tissue labeling SMAD5 with ab211141 at 1/300 dilution.
ab211141 has not yet been referenced specifically in any publications.