Product nameAnti-SMAD5 antibody
See all SMAD5 primary antibodies
DescriptionRabbit polyclonal to SMAD5
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Zebrafish
Recombinant fragment within Zebrafish SMAD5 aa 2-229. The exact sequence is proprietary.
Database link: Q9W7E7
- Zebrafish brain, heart, liver and muscle tissue extracts; zebrafish tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 1% BSA, 78% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 52 kDa.|
FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Sequence similaritiesBelongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
modificationsPhosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
- Information by UniProt
- DKFZp781C1895 antibody
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All lanes : Anti-SMAD5 antibody (ab211141) at 1/1000 dilution
Lane 1 : zebrafish brain tissue extract
Lane 2 : zebrafish heart tissue extract
Lane 3 : zebrafish liver tissue extract
Lane 4 : zebrafish muscle tissue extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
Immunohistochemical analysis of paraffin-embedded zebrafish tissue labeling SMAD5 with ab211141 at 1/300 dilution.
ab211141 has not yet been referenced specifically in any publications.