Recombinant Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)
![Western blot - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)](https://www.abcam.com/ps/products/76/ab76296/Images/ab76296-91671-anti-smad5-phospho-s463-s465-antibody-ep7282ay-western-blot.jpg "Western blot - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP728(2)AY] to SMAD5 (phospho S463 + S465)
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY]
See all SMAD5 primary antibodies -
Description
Rabbit monoclonal [EP728(2)AY] to SMAD5 (phospho S463 + S465) -
Host species
Rabbit -
Specificity
Detects SMAD5 phosphorylated at Serine 463 and 465. This antibody may cross-react with Smad1 Phospho (pS463/465) and Smad9 Phospho (pS465/467). -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
A phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human SMAD5.
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Positive control
- HeLa cytoplasmic or nuclear lysate treated with BMP-4 HeLa cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP728(2)AY -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500. Predicted molecular weight: 52 kDa.
|
| ICC/IF |
1/50 - 1/100.
|
| Notes |
|---|
|
WB
1/500. Predicted molecular weight: 52 kDa. |
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ICC/IF
1/50 - 1/100. |
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.
Target
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Function
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. - Information by UniProt
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Database links
- Entrez Gene: 4090 Human
- Omim: 603110 Human
- SwissProt: Q99717 Human
- Unigene: 167700 Human
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Alternative names
- DKFZp781C1895 antibody
- DKFZp781O1323 antibody
- Dwfc antibody
see all
Images
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Western blot - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)All lanes : Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296) at 1/500 dilution
Lane 1 : HeLa cell lysate - untreated
Lane 2 : HeLa cell lysate - treated with BMP-4
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)](https://www.abcam.com/ps/products/76/ab76296/Images/ab76296-91670-anti-smad5-phospho-s463-s465-antibody-ep7282ay-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)Immunofluorescent staining of HeLa cells using ab76296 at a 1/50-1/100 dilution. -
![Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)](https://www.abcam.com/ps/products/76/ab76296/Images/ab76296-3-benefits-of-recombinant-antibodies.png)
Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab76296 has been referenced in 4 publications.
- Zhou Q et al. A New Insight of Kartogenin Induced the Mesenchymal Stem Cells (MSCs) Selectively Differentiate into Chondrocytes by Activating the Bone Morphogenetic Protein 7 (BMP-7)/Smad5 Pathway. Med Sci Monit 25:4960-4967 (2019). PubMed: 31271564
- Cui Y et al. Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart. Cell Rep 26:1934-1950.e5 (2019). PubMed: 30759401
- Ghiabi P et al. Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche. J Transl Med 13:27 (2015). WB ; Human . PubMed: 25623554
- Nili M et al. Soluble Repulsive Guidance Molecule c/Hemojuvelin Is a Broad Spectrum Bone Morphogenetic Protein (BMP) Antagonist and Inhibits both BMP2- and BMP6-mediated Signaling and Gene Expression. J Biol Chem 285:24783-92 (2010). WB ; Human . PubMed: 20530805