Recombinant
RabMAb

Recombinant Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)

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Overview

  • Product name

    Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY]
    See all SMAD5 primary antibodies

  • Description

    Rabbit monoclonal [EP728(2)AY] to SMAD5 (phospho S463 + S465)

  • Host species

    Rabbit

  • Specificity

    Detects SMAD5 phosphorylated at Serine 463 and 465. This antibody may cross-react with Smad1 Phospho (pS463/465) and Smad9 Phospho (pS465/467).

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human SMAD5.

  • Positive control

    • HeLa cytoplasmic or nuclear lysate treated with BMP-4 HeLa cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab76296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 52 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.

    • Post-translational
      modifications

      Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.

    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
    • Information by UniProt

    • Database links

      • Alternative names

        • DKFZp781C1895 antibody
        • DKFZp781O1323 antibody
        • Dwfc antibody
        • hSmad5 antibody
        • JV5 1 antibody
        • JV5-1 antibody
        • MAD homolog 5 antibody
        • MAD, mothers against decapentaplegic homolog 5 antibody
        • MADH 5 antibody
        • MADH5 antibody
        • Mothers against decapentaplegic homolog 5 antibody
        • mothers against decapentaplegic, drosophila, homolog of, 5 antibody
        • Mothers against DPP homolog 5 antibody
        • MusMLP antibody
        • SMA and MAD related protein 5 antibody
        • SMAD 5 antibody
        • SMAD family member 5 antibody
        • SMAD, mothers against DPP homolog 5 antibody
        • Smad5 antibody
        • SMAD5_HUMAN antibody
        see all

      Images

      • Western blot - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)
        Western blot - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)
        All lanes : Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296) at 1/500 dilution

        Lane 1 : HeLa cell lysate - untreated
        Lane 2 : HeLa cell lysate - treated with BMP-4

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 52 kDa
        Observed band size: 52 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)
        Immunocytochemistry/ Immunofluorescence - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)
        Immunofluorescent staining of HeLa cells using ab76296 at a 1/50-1/100 dilution.

      References

      This product has been referenced in:

      • Zhou Q  et al. A New Insight of Kartogenin Induced the Mesenchymal Stem Cells (MSCs) Selectively Differentiate into Chondrocytes by Activating the Bone Morphogenetic Protein 7 (BMP-7)/Smad5 Pathway. Med Sci Monit 25:4960-4967 (2019). Read more (PubMed: 31271564) »
      • Cui Y  et al. Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart. Cell Rep 26:1934-1950.e5 (2019). Read more (PubMed: 30759401) »
      See all 4 Publications for this product

      Publishing research using ab76296? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Western blot abreview for Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Human prostate and cervical cancer cell)
      Gel Running Conditions
      Reduced Denaturing (7.5%)
      Loading amount
      30 µg
      Specification
      Human prostate and cervical cancer cell
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 20 2015

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