Anti-SMAD6 antibody (ab80049)
Key features and details
- Rabbit polyclonal to SMAD6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMAD6 antibody
See all SMAD6 primary antibodies -
Description
Rabbit polyclonal to SMAD6 -
Host species
Rabbit -
Specificity
This reacts specifically with human 54 kDa SMAD6 isoform D protein. It is not expected to cross react with other species. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from the C terminal domain of SMAD6 Isoform D
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Predicted molecular weight: 53 kDa.
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Notes |
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WB
1/500 - 1/5000. Predicted molecular weight: 53 kDa. |
Target
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Function
Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions. -
Tissue specificity
Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue. -
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylated by BMP type 1 receptor kinase and by PRKX.
Ubiquitinated by WWP1. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4091 Human
- Omim: 602931 Human
- SwissProt: O43541 Human
- Unigene: 153863 Human
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Alternative names
- hSMAD6 antibody
- MAD homolog 6 antibody
- MADH6 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab80049 has been referenced in 9 publications.
- Zhang M et al. The Inhibition of B7H3 by 2-HG Accumulation Is Associated With Downregulation of VEGFA in IDH Mutated Gliomas. Front Cell Dev Biol 9:670145 (2021). PubMed: 34079802
- Wang H & Ji X SMAD6, positively regulated by the DNM3OS-miR-134-5p axis, confers promoting effects to cell proliferation, migration and EMT process in retinoblastoma. Cancer Cell Int 20:23 (2020). PubMed: 31992960
- Mi B et al. Saliva exosomes-derived UBE2O mRNA promotes angiogenesis in cutaneous wounds by targeting SMAD6. J Nanobiotechnology 18:68 (2020). PubMed: 32375794
- Li M et al. GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation. Elife 9:N/A (2020). PubMed: 33006314
- Yang P et al. Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection. Biochem Res Int 2020:6285987 (2020). PubMed: 32802507
- He S et al. Circular RNA hsa_circ_0000517 modulates hepatocellular carcinoma advancement via the miR-326/SMAD6 axis. Cancer Cell Int 20:360 (2020). PubMed: 32774154
- Xu S & Wu X miR-134 inhibits chondrogenic differentiation of bone marrow mesenchymal stem cells by targetting SMAD6. Biosci Rep 39:N/A (2019). PubMed: 30135141
- Park JS et al. RON Receptor Tyrosine Kinase Regulates Epithelial Mesenchymal Transition and the Expression of Pro-Fibrotic Markers via Src/Smad Signaling in HK-2 and NRK49F Cells. Int J Mol Sci 20:N/A (2019). PubMed: 31690042
- Piacentino ML & Bronner ME Intracellular attenuation of BMP signaling via CKIP-1/Smurf1 is essential during neural crest induction. PLoS Biol 16:e2004425 (2018). PubMed: 29949573