Overview

  • Product name

  • Description

    Rabbit polyclonal to SMAD6
  • Host species

    Rabbit
  • Specificity

    This reacts specifically with human 54 kDa SMAD6 isoform D protein. It is not expected to cross react with other species.
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the C terminal domain of SMAD6 Isoform D

Properties

Applications

Our Abpromise guarantee covers the use of ab80049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/100 - 1/500.
WB 1/500 - 1/5000. Predicted molecular weight: 53 kDa.

Target

  • Function

    Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions.
  • Tissue specificity

    Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.
  • Sequence similarities

    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications

    Phosphorylated by BMP type 1 receptor kinase and by PRKX.
    Ubiquitinated by WWP1.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • hSMAD6 antibody
    • MAD homolog 6 antibody
    • MADH6 antibody
    • MADH7 antibody
    • Mothers against decapentaplegic homolog 6 antibody
    • Mothers against DPP homolog 6 antibody
    • SMAD 6 antibody
    • SMAD family member 6 antibody
    • SMAD mothers against DPP homolog 6 antibody
    • Smad6 antibody
    • SMAD6_HUMAN antibody
    see all

References

This product has been referenced in:

  • Xu S & Wu X miR-134 inhibits chondrogenic differentiation of bone marrow mesenchymal stem cells by targetting SMAD6. Biosci Rep 39:N/A (2019). Read more (PubMed: 30135141) »
  • Piacentino ML & Bronner ME Intracellular attenuation of BMP signaling via CKIP-1/Smurf1 is essential during neural crest induction. PLoS Biol 16:e2004425 (2018). Read more (PubMed: 29949573) »
See all 2 Publications for this product

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