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    smad9-antibody-ab48011.pdf

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Signal Transduction Signaling Pathway Nuclear Signaling SMADs
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Anti-SMAD9 antibody (ab48011)

  • Datasheet
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Western blot - Anti-SMAD9 antibody (ab48011)

    Key features and details

    • Goat polyclonal to SMAD9
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-SMAD9 antibody
      See all SMAD9 primary antibodies
    • Description

      Goat polyclonal to SMAD9
    • Host species

      Goat
    • Specificity

      Not expected to crossreact with SMAD1 or SMAD5.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Chicken, Dog
    • Immunogen

      Synthetic peptide:

      C-HSEPLMPHNATYPD

      , corresponding to internal sequence amino acids 166-179 of Human SMAD9.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Human skin lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.05% Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • SMADs
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • SMADs
      • Stem Cells
      • Signaling Pathways
      • TGF beta
      • Cytoplasmic
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human SMAD9 protein (ab140808)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab48011 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).

    1 hour primary incubation is recommended for this product.

    Notes
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).

    1 hour primary incubation is recommended for this product.

    Target

    • Function

      Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
    • Tissue specificity

      Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
    • Involvement in disease

      Pulmonary hypertension, primary, 2
    • Sequence similarities

      Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • Post-translational
      modifications

      Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
    • Cellular localization

      Cytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4.
    • Target information above from: UniProt accession O15198 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4093 Human
      • Entrez Gene: 55994 Mouse
      • Entrez Gene: 85435 Rat
      • Omim: 603295 Human
      • SwissProt: O15198 Human
      • SwissProt: Q9JIW5 Mouse
      • SwissProt: O54835 Rat
      • Unigene: 123119 Human
      • Unigene: 244353 Mouse
      • Unigene: 10862 Rat
      see all
    • Alternative names

      • MAD homolog 9 antibody
      • Madh6 antibody
      • Mothers against decapentaplegic antibody
      • Mothers against decapentaplegic homolog 9 antibody
      • Mothers against DPP homolog 9 antibody
      • SMAD 9 antibody
      • SMAD family member 9 antibody
      • SMAD, mothers against DPP homolog 9 (Drosophila) antibody
      • SMAD8A antibody
      • SMAD8B antibody
      • Smad9 antibody
      • SMAD9_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SMAD9 antibody (ab48011)
      Western blot - Anti-SMAD9 antibody (ab48011)
      Anti-SMAD9 antibody (ab48011) at 0.3 µg/ml + Human Skin lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 52 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab48011? Please let us know so that we can cite the reference in this datasheet.

    ab48011 has not yet been referenced specifically in any publications.

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