Anti-SMAD9 antibody (ab48011)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-SMAD9 antibody
See all SMAD9 primary antibodies -
DescriptionGoat polyclonal to SMAD9
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Host speciesGoat
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SpecificityNot expected to crossreact with SMAD1 or SMAD5.
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Tested applicationsSuitable for: Sandwich ELISA, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Dog
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Immunogen
Synthetic peptide:
C-HSEPLMPHNATYPD
, corresponding to internal sequence amino acids 166-179 of Human SMAD9. -
Positive control
- Human skin lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab48011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 1 - 2 µg/ml. Used as a reporter to detect captured recombinant SMAD9 | |
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa). |
Target
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FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
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Tissue specificityExpressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
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Involvement in diseasePulmonary hypertension, primary, 2
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Sequence similaritiesBelongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. -
Cellular localizationCytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4.
- Information by UniProt
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Database links
- Entrez Gene: 4093 Human
- Entrez Gene: 55994 Mouse
- Entrez Gene: 85435 Rat
- Omim: 603295 Human
- SwissProt: O15198 Human
- SwissProt: Q9JIW5 Mouse
- SwissProt: O54835 Rat
- Unigene: 123119 Human
see all -
Alternative names
- MAD homolog 9 antibody
- Madh6 antibody
- Mothers against decapentaplegic antibody
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Images
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Western blot - Anti-SMAD9 antibody (ab48011)Anti-SMAD9 antibody (ab48011) at 0.3 µg/ml + Human Skin lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
ab48011 has not yet been referenced specifically in any publications.
