Anti-Smallpox B5R antibody (ab53309)
Key features and details
- Rabbit polyclonal to Smallpox B5R
- Suitable for: WB
- Reacts with: Variola virus
- Isotype: IgG
Overview
-
Product name
Anti-Smallpox B5R antibody -
Description
Rabbit polyclonal to Smallpox B5R -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Variola virus -
Immunogen
Synthetic peptide corresponding to Smallpox B5R aa 180-194. Variola virus
Sequence:HYKKSESESYNELIK
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.2% Gelatin, PBS -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 64 kDa).
|
Notes |
---|
WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 64 kDa). |
Target
-
Relevance
B5R is an EEV-specific Type I membrane glycoprotein essential for EEV formation. Vaccinia virus, a member of Poxviridae family, is a dsDNA enveloped virus. It consists of 4 types of virion stages among which extracellular enveloped virus (EEV) is critical for cell-to-cell and long range viral infection. Some cellular antigens are associated with EEV and these vary with the cell type used to grow the virus. B5R consists of a large ectodomain containing regions similar to SCR domains of complement regulatory proteins followed by a transmembrane region and a cytoplasmic tail. B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, and for EEV formation. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 3707658 Variola virus
- SwissProt: B1PHX2 Variola virus
- SwissProt: B2BAB2 Variola virus
- SwissProt: Q76ZL8 Variola virus
-
Alternative names
- B5R antibody
- EEV type I membrane glycoprotein antibody
- Smallpox antibody
Images
-
All lanes : Anti-Smallpox B5R antibody (ab53309) at 0.1 µg/ml
Lane 1 : recombinant fusion protein containing amino acids 180-194
Lane 2 : fusion partner only
Predicted band size: 64 kDa
Observed band size: 30,60 kDa why is the actual band size different from the predicted?
60kDa band is a dimerized peptide carrier.
Datasheets and documents
-
Datasheet download
References (0)
ab53309 has not yet been referenced specifically in any publications.