Key features and details
- Rabbit polyclonal to Smallpox B5R
- Suitable for: WB
- Isotype: IgG
Product nameAnti-Smallpox B5R antibody
DescriptionRabbit polyclonal to Smallpox B5R
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Other species
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.2% Gelatin, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab53309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 64 kDa).|
RelevanceB5R is an EEV-specific Type I membrane glycoprotein essential for EEV formation. Vaccinia virus, a member of Poxviridae family, is a dsDNA enveloped virus. It consists of 4 types of virion stages among which extracellular enveloped virus (EEV) is critical for cell-to-cell and long range viral infection. Some cellular antigens are associated with EEV and these vary with the cell type used to grow the virus. B5R consists of a large ectodomain containing regions similar to SCR domains of complement regulatory proteins followed by a transmembrane region and a cytoplasmic tail. B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, and for EEV formation.
Cellular localizationCell Membrane
- B5R antibody
- EEV type I membrane glycoprotein antibody
- Smallpox antibody
All lanes : Anti-Smallpox B5R antibody (ab53309) at 0.1 µg/ml
Lane 1 : recombinant fusion protein containing amino acids 180-194
Lane 2 : fusion partner only
Predicted band size: 64 kDa
Observed band size: 30,60 kDa why is the actual band size different from the predicted?
60kDa band is a dimerized peptide carrier.
ab53309 has not yet been referenced specifically in any publications.