Overview

  • Product name

    Anti-Smallpox B5R antibody
  • Description

    Rabbit polyclonal to Smallpox B5R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Synthetic peptide corresponding to Smallpox B5R aa 180-194. Variola virus
    Sequence:

    HYKKSESESYNELIK

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.2% Gelatin, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 64 kDa).

Target

  • Relevance

    B5R is an EEV-specific Type I membrane glycoprotein essential for EEV formation. Vaccinia virus, a member of Poxviridae family, is a dsDNA enveloped virus. It consists of 4 types of virion stages among which extracellular enveloped virus (EEV) is critical for cell-to-cell and long range viral infection. Some cellular antigens are associated with EEV and these vary with the cell type used to grow the virus. B5R consists of a large ectodomain containing regions similar to SCR domains of complement regulatory proteins followed by a transmembrane region and a cytoplasmic tail. B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, and for EEV formation.
  • Cellular localization

    Cell Membrane
  • Alternative names

    • B5R antibody
    • EEV type I membrane glycoprotein antibody
    • Smallpox antibody

Images

  • All lanes : Anti-Smallpox B5R antibody (ab53309) at 0.1 µg/ml

    Lane 1 : recombinant fusion protein containing amino acids 180-194
    Lane 2 : fusion partner only

    Predicted band size: 64 kDa
    Observed band size: 30,60 kDa
    why is the actual band size different from the predicted?



    60kDa band is a dimerized peptide carrier.

References

ab53309 has not yet been referenced specifically in any publications.

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