Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.2% Gelatin, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab53310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 30 kDa.|
RelevanceB5R is an EEV-specific Type I membrane glycoprotein essential for EEV formation. Vaccinia virus, a member of Poxviridae family, is a dsDNA enveloped virus. It consists of 4 types of virion stages among which extracellular enveloped virus (EEV) is critical for cell-to-cell and long range viral infection. Some cellular antigens are associated with EEV and these vary with the cell type used to grow the virus. B5R consists of a large ectodomain containing regions similar to SCR domains of complement regulatory proteins followed by a transmembrane region and a cytoplasmic tail. B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, and for EEV formation.
Cellular localizationCell Membrane
- B5R antibody
- EEV type I membrane glycoprotein antibody
- Smallpox antibody
All lanes : Anti-Smallpox B5R antibody - Aminoterminal end (ab53310) at 0.1 µg/ml
Lane 1 : recombinant fusion protein containing amino acids 33-47
Lane 2 : fusion partner only
Observed band size: 30,60 kDa why is the actual band size different from the predicted?
60kDa band is the dimerized peptide carrier.
ab53310 has not yet been referenced specifically in any publications.