Key features and details
- Rabbit polyclonal to SMAP
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SMAP antibody
See all SMAP primary antibodies
DescriptionRabbit polyclonal to SMAP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
Synthetic peptide within Human SMAP aa 1-50. The exact sequence is proprietary. NP_055082.1
Database link: O00193
- Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab202282 was affinity purified using an epitope specific to SMAP immobilized on solid support.
Our Abpromise guarantee covers the use of ab202282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the SMAP family.
- Information by UniProt
- C11orf58 antibody
- chromosome 11 open reading frame 58 antibody
- DKFZp686N0232 antibody
All lanes : Anti-SMAP antibody (ab202282) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells
Lane 2 : Whole cell lysate from 293T cells
Lane 3 : Whole cell lysate from Jurkat cells
Lane 4 : Whole cell lysate from mouse TCMK-1 cells
Lane 5 : Whole cell lysate from mouse NIH 3T3 cells
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
Lane 1: Detection of Human SMAP by Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202282 at 6 µg per reaction for IP.
Lane 2: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognized an epitope of SMAP.
Lane 3: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab202282 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 30
ab202282 has not yet been referenced specifically in any publications.