Key features and details
- Rabbit polyclonal to SMAP2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SMAP2 antibody
DescriptionRabbit polyclonal to SMAP2
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
antigen sequence, corresponding to C terminal amino acids 326-429 of Human SMAP2.
- Human lymph node tissue; A431 cells; RT 4, U 251 MG, Human Liver and Tonsil cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionGTPase activating protein that acts on ARF1. Can also activate ARF6 (in vitro). May play a role in clathrin-dependent retrograde transport from early endosomes to the trans-Golgi network.
Sequence similaritiesContains 1 Arf-GAP domain.
Cellular localizationCytoplasm. Detected in multiple foci throughout the cytoplasm and in juxtanuclear structures.
- Information by UniProt
- RP1-228H13.3 antibody
- Small ArfGAP2 antibody
- SMAP 1L antibody
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
All lanes : Anti-SMAP2 antibody (ab121675) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab121675 at 1/300 dilution staining SMAP2 in Paraffin-embedded Human lymph node tissue by Immunohistochemistry.
ab121675 at 4 µg/ml staining SMAP2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
ab121675 has not yet been referenced specifically in any publications.