Product nameAnti-SMARCA2 / BRM antibody
See all SMARCA2 / BRM primary antibodies
DescriptionRabbit polyclonal to SMARCA2 / BRM
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chicken, Cow
Synthetic peptide within Human SMARCA2/ BRM (N terminal). The exact sequence is proprietary.
Database link: P51531
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900). ICC: HeLa cells. IHC-P: Mouse brain and testis tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 181 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionTranscriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Involvement in diseaseNicolaides-Baraitser syndrome
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 1 bromo domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HSA domain.
Contains 1 QLQ domain.
- Information by UniProt
- ATP dependent helicase SMARCA2 antibody
- ATP-dependent helicase SMARCA2 antibody
- BAF190 antibody
All lanes : Anti-SMARCA2 / BRM antibody (ab227000) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 181 kDa
Paraffin embedded mouse brain tissue stained for SMARCA2 / BRM using ab227000 at 1/500 dilution in immunohistochemical analysis.
Paraffin embedded mouse testis tissue stained for SMARCA2 / BRM using ab227000 at 1/500 dilution in immunohistochemical analysis.
4% paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SMARCA2 / BRM using ab227000 at 1/500 dilution in ICC/IF.
ab227000 has not yet been referenced specifically in any publications.