Anti-SMARCA2 / BRM antibody (ab264164)
Key features and details
- Rabbit polyclonal to SMARCA2 / BRM
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMARCA2 / BRM antibody
See all SMARCA2 / BRM primary antibodies -
Description
Rabbit polyclonal to SMARCA2 / BRM -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human SMARCA2/ BRM aa 100-150. The exact sequence is proprietary.
Database link: P51531 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. -
Involvement in disease
Nicolaides-Baraitser syndrome -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Contains 1 bromo domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HSA domain.
Contains 1 QLQ domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6595 Human
- Entrez Gene: 67155 Mouse
- Omim: 600014 Human
- SwissProt: P51531 Human
- SwissProt: Q6DIC0 Mouse
- Unigene: 298990 Human
- Unigene: 644901 Human
- Unigene: 491146 Mouse
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Alternative names
- ATP dependent helicase SMARCA2 antibody
- ATP-dependent helicase SMARCA2 antibody
- BAF190 antibody
see all
Images
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All lanes : Anti-SMARCA2 / BRM antibody (ab264164) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Exposure time: 10 seconds4-8% SDS-PAGE
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SMARCA2 / BRM was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264164 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264164 at 1 µg/ml.
Lane 1: ab264164 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 secs.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab264164 has not yet been referenced specifically in any publications.