Product nameAnti-SMARCA2 / BRM antibody
See all SMARCA2 / BRM primary antibodies
DescriptionRabbit polyclonal to SMARCA2 / BRM
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human SMARCA2/ BRM aa 100-150. The exact sequence is proprietary.
Database link: P51531
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264164 in the following tested applications.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionTranscriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Involvement in diseaseNicolaides-Baraitser syndrome
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 1 bromo domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HSA domain.
Contains 1 QLQ domain.
- Information by UniProt
- ATP dependent helicase SMARCA2 antibody
- ATP-dependent helicase SMARCA2 antibody
- BAF190 antibody
All lanes : Anti-SMARCA2 / BRM antibody (ab264164) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Exposure time: 10 seconds
SMARCA2 / BRM was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264164 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264164 at 1 µg/ml.
Lane 1: ab264164 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
ab264164 has not yet been referenced specifically in any publications.