Anti-SMARCA6 antibody (ab3851)
Key features and details
- Rabbit polyclonal to SMARCA6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMARCA6 antibody
See all SMARCA6 primary antibodies -
Description
Rabbit polyclonal to SMARCA6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (Human) - which represents a portion of human Helicase Lymphoid Specific encoded in part by exons 15 and 16.
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Positive control
- WB: HeLa whole cell lysate
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General notes
DNA helicases (Hel) play a role in a number of processes involving DNA strand separation, including replication, repair, recombination, and transcription. Jarvis et al. (1996) identified a mouse gene encoding a novel putative helicase. They designated the gene LSH, for lymphoid-specific Hel, because Northern blot analysis revealed that it was expressed specifically in mouse early thymocytes. Geiman et al. (1998) determined that the mouse LSH, or HELLS (helicase lymphoid-specific), gene contains at least 18 exons and spans more than 26.6 kb. Using Northern blot analysis, these authors found that human HELLS is expressed in testis and thymus, two tissues that are highly active in DNA recombination. Geiman and Muegge (2000) demonstrated that LSH is not obligatory for normal lymphoid development but is essential for normal proliferation of peripheral T lymphocytes.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
DNA helicases (Hel) play a role in a number of processes involving DNA strand separation, including replication, repair, recombination, and transcription. Jarvis et al. (1996) identified a mouse gene encoding a novel putative helicase. They designated the gene LSH, for lymphoid-specific Hel, because Northern blot analysis revealed that it was expressed specifically in mouse early thymocytes. Geiman et al. (1998) determined that the mouse LSH, or HELLS (helicase lymphoid-specific), gene contains at least 18 exons and spans more than 26.6 kb. Using Northern blot analysis, these authors found that human HELLS is expressed in testis and thymus, two tissues that are highly active in DNA recombination. Geiman and Muegge (2000) demonstrated that LSH is not obligatory for normal lymphoid development but is essential for normal proliferation of peripheral T lymphocytes. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
Target
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Function
Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis. -
Tissue specificity
Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. Closely associated with pericentric heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 3070 Human
- GenBank: AAH30963 Human
- Omim: 603946 Human
- SwissProt: Q9NRZ9 Human
- Unigene: 655830 Human
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Alternative names
- FLJ10339 antibody
- Helicase lymphoid specific antibody
- Hells antibody
see all
Images
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All lanes : Anti-SMARCA6 antibody (ab3851) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 97 kDa
Exposure time: 3 minutes -
Anti-SMARCA6 antibody (ab3851) at 1 µg/ml + Human thymus tissue lysate - total protein (ab30146) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 102 kDa, 120 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab3851 has been referenced in 2 publications.
- He C & Liu L Hsa_circ_0072008 regulates cell proliferation, migration, and invasion in cervical squamous cell carcinoma via miR-1305/helicase, lymphoid specific (HELLS) axis. Bioengineered 13:8311-8322 (2022). PubMed: 35311456
- Jenness C et al. HELLS and CDCA7 comprise a bipartite nucleosome remodeling complex defective in ICF syndrome. Proc Natl Acad Sci U S A 115:E876-E885 (2018). PubMed: 29339483