Anti-SmarcAL1 antibody (ab154226)
- Datasheet
- Specific References (1)
- Protocols
Overview
-
Product nameAnti-SmarcAL1 antibody
See all SmarcAL1 primary antibodies -
DescriptionRabbit polyclonal to SmarcAL1
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, ICC/IFmore details
-
Species reactivityReacts with: Human
-
Immunogen
Recombinant fragment corresponding to a region within amino acids 120 and 457 of Human SmarcAL1 (UniProt ID: Q9NZC9)
-
Positive control
- A549 whole cell lysate, A549 cells.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 106 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
FunctionATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA.
-
Tissue specificityUbiquitously expressed, with high levels in testis.
-
Involvement in diseaseDefects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD) [MIM:242900]. SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema.
-
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.
Contains 2 HARP domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localizationNucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 50485 Human
- Omim: 606622 Human
- SwissProt: Q9NZC9 Human
- Unigene: 516674 Human
-
Alternative names
- HARP antibody
- HepA Related Protein antibody
- HepA-related protein antibody
see all
Images
-
Western blot - Anti-SmarcAL1 antibody (ab154226)Anti-SmarcAL1 antibody (ab154226) at 1/500 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 106 kDa
5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SmarcAL1 antibody (ab154226)Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, labeling SmarcAL1 with ab154226 at a 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image).
References
This product has been referenced in:
- Keka IS et al. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining. Nucleic Acids Res N/A:N/A (2015). Human . Read more (PubMed: 26089390) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"