Anti-SmarcAL1 antibody (ab154226)
Key features and details
- Rabbit polyclonal to SmarcAL1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SmarcAL1 antibody
See all SmarcAL1 primary antibodies -
Description
Rabbit polyclonal to SmarcAL1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment corresponding to a region within amino acids 120 and 457 of Human SmarcAL1 (UniProt ID: Q9NZC9)
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Positive control
- A549 whole cell lysate, A549 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 106 kDa.
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 106 kDa. |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. -
Tissue specificity
Ubiquitously expressed, with high levels in testis. -
Involvement in disease
Defects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD) [MIM:242900]. SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.
Contains 2 HARP domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 50485 Human
- Omim: 606622 Human
- SwissProt: Q9NZC9 Human
- Unigene: 516674 Human
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Alternative names
- HARP antibody
- HepA Related Protein antibody
- HepA-related protein antibody
see all
Images
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Western blot - Anti-SmarcAL1 antibody (ab154226)Anti-SmarcAL1 antibody (ab154226) at 1/500 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 106 kDa
5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SmarcAL1 antibody (ab154226)Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, labeling SmarcAL1 with ab154226 at a 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image).
Protocols
References (2)
ab154226 has been referenced in 2 publications.
- Pugliese GM et al. Inducible SMARCAL1 knockdown in iPSC reveals a link between replication stress and altered expression of master differentiation genes. Dis Model Mech 12:N/A (2019). PubMed: 31515241
- Keka IS et al. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining. Nucleic Acids Res N/A:N/A (2015). Human . PubMed: 26089390
