Anti-SmarcAL1 antibody (ab154226)

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Overview

  • Product name

    Anti-SmarcAL1 antibody
    See all SmarcAL1 primary antibodies

  • Description

    Rabbit polyclonal to SmarcAL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 120 and 457 of Human SmarcAL1 (UniProt ID: Q9NZC9)

  • Positive control

    • A549 whole cell lysate, A549 cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 106 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA.

  • Tissue specificity

    Ubiquitously expressed, with high levels in testis.

  • Involvement in disease

    Defects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD) [MIM:242900]. SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema.

  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.
    Contains 2 HARP domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • HARP antibody
      • HepA Related Protein antibody
      • HepA-related protein antibody
      • hHARP antibody
      • SIOD antibody
      • SMAL1_HUMAN antibody
      • SMARCA like Protein 1 antibody
      • smarcal1 antibody
      • Sucrose nonfermenting protein 2 like 1 antibody
      • Sucrose nonfermenting protein 2-like 1 antibody
      • SWI/SNF Related antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A like protein 1 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 antibody
      see all

    Images

    • Western blot - Anti-SmarcAL1 antibody (ab154226)
      Western blot - Anti-SmarcAL1 antibody (ab154226)
      Anti-SmarcAL1 antibody (ab154226) at 1/500 dilution + A549 whole cell lysate at 30 µg

      Predicted band size: 106 kDa



      5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-SmarcAL1 antibody (ab154226)
      Immunocytochemistry/ Immunofluorescence - Anti-SmarcAL1 antibody (ab154226)
      Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, labeling SmarcAL1 with ab154226 at a 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image).

    References

    This product has been referenced in:

    • Keka IS  et al. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining. Nucleic Acids Res N/A:N/A (2015). Human . Read more (PubMed: 26089390) »
    See 1 Publication for this product

    Publishing research using ab154226? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-SmarcAL1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa)
    Total protein in input
    2e+006 cells
    Immuno-precipitation step
    Protein G
    Specification
    HeLa
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 17 2017

    Western blot abreview for Anti-SmarcAL1 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (Hek 293T)
    Gel Running Conditions
    Reduced Denaturing (6%)
    Loading amount
    1e+007 cells
    Specification
    Hek 293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 21 2016

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