Key features and details
- Rabbit polyclonal to SmarcAL1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SmarcAL1 antibody
See all SmarcAL1 primary antibodies
DescriptionRabbit polyclonal to SmarcAL1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human SmarcAL1 aa 6-277.
TEEQRKKIEENRQKALARRAEKLLAEQHQRTSSGTSIAGNPFQAKQGPSQ NFPRESCKPVSHGVIFKQQNLSSSSNADQRPHDSHSFQAKGIWKKPEEMP TACPGHSPRSQMALTGISPPLAQSPPEVPKQQLLSYELGQGHAQASPEIR FTPFANPTHKPLAKPKSSQETPAHSSGQPPRDAKLEAKTAKASPSGQNIS YIHSSSESVTPRTEGRLQQKSGSSVQKGVNSQKGKCVRNGDRFQVLIGYN AELIAVFKTLPSKNYDPDTKTW
Database link: Q9NZC9
- WB: Jurkat whole cell lysate; Mouse lung and kidney tissue lysate. ICC/IF: HeLa cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|ICC/IF||1/50 - 1/200.|
FunctionATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA.
Tissue specificityUbiquitously expressed, with high levels in testis.
Involvement in diseaseDefects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD) [MIM:242900]. SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.
Contains 2 HARP domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
- Information by UniProt
- HARP antibody
- HepA Related Protein antibody
- HepA-related protein antibody
All lanes : Anti-SmarcAL1 antibody (ab236786) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse kidney tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SmarcAL1 (green) using ab236786 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236786 has not yet been referenced specifically in any publications.