Recombinant
RabMAb

Anti-SMARCC1 / BAF155 antibody [EPR12389] (ab172636)

Overview

  • Product name
    Anti-SMARCC1 / BAF155 antibody [EPR12389]
    See all SMARCC1 / BAF155 primary antibodies
  • Description
    Rabbit monoclonal [EPR12389] to SMARCC1 / BAF155
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SMARCC1/ BAF155 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q92922

  • Positive control
    • NTera2/D1, HeLa and Jurkat cell lysates. HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as SMARCC1. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
    • Tissue specificity
      Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.
    • Sequence similarities
      Belongs to the SMARCC family.
      Contains 1 SANT domain.
      Contains 1 SWIRM domain.
    • Post-translational
      modifications
      Phosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI115498 antibody
      • BAF 155 antibody
      • BAF155 antibody
      • BRG 1 associated factor 155 antibody
      • BRG1 associated factor 155 antibody
      • BRG1-associated factor 155 antibody
      • Chromatin remodeling complex BAF155 subunit antibody
      • CRACC 1 antibody
      • CRACC1 antibody
      • Mammalian chromatin remodeling complex BRG 1 associated factor 155 antibody
      • Mammalian chromatin remodeling complex BRG1 associated factor 155 antibody
      • Rsc 8 antibody
      • Rsc8 antibody
      • SMARC C1 antibody
      • SMARCC 1 antibody
      • SMARCC1 antibody
      • SMRC1_HUMAN antibody
      • SRG 3 antibody
      • SRG3 antibody
      • SWI 3 antibody
      • SWI/SNF complex 155 kDa subunit antibody
      • SWI/SNF complex subunit SMARCC1 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin c1 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 antibody
      • SWI3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling SMARCC1 / BAF155 with ab172636 at a 1/100 dilution.

    • All lanes : Anti-SMARCC1 / BAF155 antibody [EPR12389] (ab172636) at 1/1000 dilution

      Lane 1 : NTera2/D1 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 123 kDa

    References

    ab172636 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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