Recombinant
RabMAb

Anti-SMARCC1 / BAF155 antibody [EPR12395] (ab172638)

Overview

  • Product name
    Anti-SMARCC1 / BAF155 antibody [EPR12395]
    See all SMARCC1 / BAF155 primary antibodies
  • Description
    Rabbit monoclonal [EPR12395] to SMARCC1 / BAF155
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human SMARCC1/ BAF155 aa 700-800 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q92922

  • Positive control
    • HeLa, K562, Jurkat and 293T cell lysates. Permeabilized Jurkat cells. Immunoprecipitation pellet from Jurkat cell lysate. Rat testis lysates.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as SMARCC1. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
ICC/IF 1/100.

For unpurified use at 1/250 - 1/500. 

IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Tissue specificity
    Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the SMARCC family.
    Contains 1 SANT domain.
    Contains 1 SWIRM domain.
  • Post-translational
    modifications
    Phosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI115498 antibody
    • BAF 155 antibody
    • BAF155 antibody
    • BRG 1 associated factor 155 antibody
    • BRG1 associated factor 155 antibody
    • BRG1-associated factor 155 antibody
    • Chromatin remodeling complex BAF155 subunit antibody
    • CRACC 1 antibody
    • CRACC1 antibody
    • Mammalian chromatin remodeling complex BRG 1 associated factor 155 antibody
    • Mammalian chromatin remodeling complex BRG1 associated factor 155 antibody
    • Rsc 8 antibody
    • Rsc8 antibody
    • SMARC C1 antibody
    • SMARCC 1 antibody
    • SMARCC1 antibody
    • SMRC1_HUMAN antibody
    • SRG 3 antibody
    • SRG3 antibody
    • SWI 3 antibody
    • SWI/SNF complex 155 kDa subunit antibody
    • SWI/SNF complex subunit SMARCC1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin c1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 antibody
    • SWI3 antibody
    see all

Images

  • All lanes : Anti-SMARCC1 / BAF155 antibody [EPR12395] (ab172638) at 1/5000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Rat testis lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 123 kDa
    Observed band size: 155 kDa (why is the actual band size different from the predicted?)



    Blocking and diluting buffer: 5% NFDM/TBST.

  • ab172638 (purified) at 1:30 dilution (2μg) immunoprecipitating SMARCC1 / BAF155 in Jurkat whole cell lysate.

    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, 10μg
    Lane 2 (+): ab172638 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab172638 in Jurkat whole cell lysate

    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST."

  • Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling SMARCC1 / BAF155 with purified ab172638 at 1:30 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SMARCC2 with Purified ab172638 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using unpurified ab172638 at a 1/10 dilution.

  • Flow cytometric analysis of permeabilized Jurkat cells using unpurified ab172638 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • All lanes : Anti-SMARCC1 / BAF155 antibody [EPR12395] (ab172638) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 123 kDa

References

This product has been referenced in:
  • Scott CA  et al. Nuclear/cytoplasmic transport defects in BBS6 underlie congenital heart disease through perturbation of a chromatin remodeling protein. PLoS Genet 13:e1006936 (2017). Read more (PubMed: 28753627) »
  • Cabot B  et al. Differential expression of key subunits of SWI/SNF chromatin remodeling complexes in porcine embryos derived in vitro or in vivo. Mol Reprod Dev 84:1238-1249 (2017). ICC/IF . Read more (PubMed: 29024220) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (whole embryo/epiboly stage)
Permeabilization
Yes - water and PBS-Triton
Specification
whole embryo/epiboly stage
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Charles Scott

Verified customer

Submitted Jan 15 2015

Application
Western blot
Sample
Zebrafish Tissue lysate - nuclear (whole embryo lysate Cyto/Nuc fractionation)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
whole embryo lysate Cyto/Nuc fractionation
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Charles Scott

Verified customer

Submitted Dec 11 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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