Anti-SMARCC1/BAF155 antibody (ab126180)
Key features and details
- Rabbit polyclonal to SMARCC1/BAF155
- Suitable for: WB, IHC-P, ICC/IF, ChIP, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SMARCC1/BAF155 antibody
See all SMARCC1/BAF155 primary antibodies -
Description
Rabbit polyclonal to SMARCC1/BAF155 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, ChIP, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human SMARCC1/BAF155 aa 808-1052 (C terminal).
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Positive control
- ChIP: MCF7 chromatin extract; WB: HeLa, Neuro-2a, C8-D30, NIH/3T3, RAW 264.7, C2C12, HepG2, A431, HEK-293T lysate; IHC-P: BT483, RT2 and C2C12 xenograft, rat hind brain, mouse prostate and duodenum; IF: HeLa whole cells; IP: HEK-293T lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/20000. Predicted molecular weight: 123 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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ChIP |
Use at an assay dependent concentration.
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IP |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/20000. Predicted molecular weight: 123 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
ChIP
Use at an assay dependent concentration. |
IP
1/100 - 1/500. |
Target
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Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. -
Tissue specificity
Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. -
Sequence similarities
Belongs to the SMARCC family.
Contains 1 SANT domain.
Contains 1 SWIRM domain. -
Post-translational
modificationsPhosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6599 Human
- Entrez Gene: 20588 Mouse
- Entrez Gene: 301020 Rat
- Omim: 601732 Human
- SwissProt: Q92922 Human
- SwissProt: P97496 Mouse
- Unigene: 476179 Human
- Unigene: 85410 Mouse
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Alternative names
- AI115498 antibody
- BAF 155 antibody
- BAF155 antibody
see all
Images
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Cross-linked ChIP was performed with MCF7 (human breast adenocarcinoma cell line) chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 μg of either control rabbit IgG or ab126180. The precipitated DNA was detected by PCR with primer set targeting to ESR1 or Sat2.
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All lanes : Anti-SMARCC1/BAF155 antibody (ab126180) at 1/5000 dilution
Lane 1 : Wild-Type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : SMARCC1/BAF155 Knockout HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 123 kDa5% SDS-PAGE
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All lanes : Anti-SMARCC1/BAF155 antibody (ab126180) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 123 kDa5% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded human BT483 xenograft staining SMARCC1/BAF155 protein using ab126180 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labeling SMARCC1/BAF155 protein (green) with ab126180 at 1/500 dilution. Red: phalloidin, a cytoskeleton marker, at 1/200 dilution. Scale bar= 10 μm.
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ab126180 immunoprecipitates SMARCC1 protein using HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.
IP Sample:
1- HEK-293T whole cell lysate, 30 μg;
2- Control with 2.5 μg of pre-immune rabbit IgG;
3- Immunoprecipitation of SMARCC1 by 2.5 μg of ab126180.
5% SDS-PAGE
The immunoprecipitated SMARCC1 protein was detected byab126180 at 1/1000 dilution, followed by anti-rabbit IgG (HRP) antibody.
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Immunohistochemical analysis of paraffin-embedded mouse prostate staining SMARCC1/BAF155 protein at nucleus using ab126180 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunohistochemical analysis of paraffin-embedded mouse duodenum staining SMARCC1/BAF155 protein at nucleus using ab126180 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunohistochemical analysis of paraffin-embedded rat hind brain staining SMARCC1/BAF155 protein at nucleus using ab126180 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labeling SMARCC1/BAF155 protein (green) with ab126180 at 1/500 dilution. Red: Alpha-tubulin filaments, at 1/2000 dilution.
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All lanes : Anti-SMARCC1/BAF155 antibody (ab126180) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 123 kDa5% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded C2C12 (mouse myoblast cell line) xenograft staining SMARCC1/BAF155 protein using ab126180 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunohistochemical analysis of paraffin-embedded RT2 xenograft staining SMARCC1/BAF155 protein using ab126180 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab126180 has been referenced in 1 publication.
- Xiao ZM et al. SMARCC1 Suppresses Tumor Progression by Inhibiting the PI3K/AKT Signaling Pathway in Prostate Cancer. Front Cell Dev Biol 9:678967 (2021). PubMed: 34249931