Product nameAnti-SMARCC1/BAF155 antibody
See all SMARCC1/BAF155 primary antibodies
DescriptionRabbit polyclonal to SMARCC1/BAF155
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human SMARCC1/BAF155 aa 300-350. The exact sequence is proprietary.
Database link: Q92922
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264166 in the following tested applications.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000.|
FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Tissue specificityExpressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.
Sequence similaritiesBelongs to the SMARCC family.
Contains 1 SANT domain.
Contains 1 SWIRM domain.
modificationsPhosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein.
- Information by UniProt
- AI115498 antibody
- BAF 155 antibody
- BAF155 antibody
All lanes : Anti-SMARCC1/BAF155 antibody (ab264166) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Exposure time: 30 seconds
SMARCC1/BAF155 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264166 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264166 at 1 µg/ml.
Lane 1: ab264166 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264166 has not yet been referenced specifically in any publications.