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    smarcc1baf155-antibody-ab72503.pdf

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Epigenetics and Nuclear Signaling Chromatin Remodeling Swi / Snf
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Anti-SMARCC1/BAF155 antibody (ab72503)

  • Datasheet
  • SDS
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Western blot - Anti-SMARCC1/BAF155 antibody (ab72503)
  • Immunoprecipitation - Anti-SMARCC1/BAF155 antibody (ab72503)
  • Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody (ab72503)

Key features and details

  • Rabbit polyclonal to SMARCC1/BAF155
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SMARCC1/BAF155 antibody
    See all SMARCC1/BAF155 primary antibodies
  • Description

    Rabbit polyclonal to SMARCC1/BAF155
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human SMARCC1/BAF155 (C terminal).
    Database link: NP_003065.2

  • Positive control

    • HeLa whole cell lysate (ab150035).
  • General notes

    Previously labelled as SMARCC1. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab72503 was affinity purified using an epitope specific to SMARCC1/BAF155 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Swi / Snf
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab72503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IP
Human
WB
Human
All applications
Horse
Dog
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Elephant
Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 123 kDa).
IP
Use at 2-5 µg/mg of lysate.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 123 kDa).
IP
Use at 2-5 µg/mg of lysate.

Target

  • Function

    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Tissue specificity

    Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the SMARCC family.
    Contains 1 SANT domain.
    Contains 1 SWIRM domain.
  • Post-translational
    modifications

    Phosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q92922 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6599 Human
    • Omim: 601732 Human
    • SwissProt: Q92922 Human
    • Unigene: 476179 Human
    • Alternative names

      • AI115498 antibody
      • BAF 155 antibody
      • BAF155 antibody
      • BRG 1 associated factor 155 antibody
      • BRG1 associated factor 155 antibody
      • BRG1-associated factor 155 antibody
      • Chromatin remodeling complex BAF155 subunit antibody
      • CRACC 1 antibody
      • CRACC1 antibody
      • Mammalian chromatin remodeling complex BRG 1 associated factor 155 antibody
      • Mammalian chromatin remodeling complex BRG1 associated factor 155 antibody
      • Rsc 8 antibody
      • Rsc8 antibody
      • SMARC C1 antibody
      • SMARCC 1 antibody
      • SMARCC1 antibody
      • SMRC1_HUMAN antibody
      • SRG 3 antibody
      • SRG3 antibody
      • SWI 3 antibody
      • SWI/SNF complex 155 kDa subunit antibody
      • SWI/SNF complex subunit SMARCC1 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin c1 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 antibody
      • SWI3 antibody
      see all

    Images

    • Western blot - Anti-SMARCC1/BAF155 antibody (ab72503)
      Western blot - Anti-SMARCC1/BAF155 antibody (ab72503)
      All lanes : Anti-SMARCC1/BAF155 antibody (ab72503) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Predicted band size: 123 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?


      Exposure time: 1 second
    • Immunoprecipitation - Anti-SMARCC1/BAF155 antibody (ab72503)
      Immunoprecipitation - Anti-SMARCC1/BAF155 antibody (ab72503)

      Detection of SMARCC1/BAF155 by Western Blot of Immunprecipitate.
      ab72503 at 1µg/ml staining SMARCC1/BAF155 in HeLa whole cell lysate immunoprecipitated using ab72503 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 1 second.

    • Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody (ab72503)
      Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody (ab72503)
      ICC/IF image of ab72503 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72503, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab72503? Please let us know so that we can cite the reference in this datasheet.

    ab72503 has been referenced in 1 publication.

    • Chen L  et al. Transcriptional control in cardiac progenitors: Tbx1 interacts with the BAF chromatin remodeling complex and regulates Wnt5a. PLoS Genet 8:e1002571 (2012). WB ; Mouse . PubMed: 22438823

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