Anti-SMARCC1/BAF155 antibody (ab72503)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-SMARCC1/BAF155 antibody
See all SMARCC1/BAF155 primary antibodies -
Description
Rabbit polyclonal to SMARCC1/BAF155 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to Human SMARCC1/BAF155 (C terminal).
Database link: NP_003065.2 -
Positive control
- HeLa whole cell lysate (ab150035).
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General notes
Previously labelled as SMARCC1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab72503 was affinity purified using an epitope specific to SMARCC1/BAF155 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab72503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 123 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
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Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. -
Tissue specificity
Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. -
Sequence similarities
Belongs to the SMARCC family.
Contains 1 SANT domain.
Contains 1 SWIRM domain. -
Post-translational
modificationsPhosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6599 Human
- Entrez Gene: 20588 Mouse
- Omim: 601732 Human
- SwissProt: Q92922 Human
- SwissProt: P97496 Mouse
- Unigene: 476179 Human
- Unigene: 85410 Mouse
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Alternative names
- AI115498 antibody
- BAF 155 antibody
- BAF155 antibody
see all
Images
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Western blot - Anti-SMARCC1/BAF155 antibody (ab72503)All lanes : Anti-SMARCC1/BAF155 antibody (ab72503) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 123 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 1 second -
Immunoprecipitation - Anti-SMARCC1/BAF155 antibody (ab72503)Detection of SMARCC1/BAF155 by Western Blot of Immunprecipitate.
ab72503 at 1µg/ml staining SMARCC1/BAF155 in HeLa whole cell lysate immunoprecipitated using ab72503 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 1 second. -
Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody (ab72503)ICC/IF image of ab72503 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72503, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Chen L et al. Transcriptional control in cardiac progenitors: Tbx1 interacts with the BAF chromatin remodeling complex and regulates Wnt5a. PLoS Genet 8:e1002571 (2012). WB ; Mouse . Read more (PubMed: 22438823) »
