Anti-SMARCC1/BAF155 antibody (ab72503)
Key features and details
- Rabbit polyclonal to SMARCC1/BAF155
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMARCC1/BAF155 antibody
See all SMARCC1/BAF155 primary antibodies -
Description
Rabbit polyclonal to SMARCC1/BAF155 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human SMARCC1/BAF155 (C terminal).
Database link: NP_003065.2 -
Positive control
- HeLa whole cell lysate (ab150035).
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General notes
Previously labelled as SMARCC1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab72503 was affinity purified using an epitope specific to SMARCC1/BAF155 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IP |
Human
|
| WB |
Human
|
| All applications |
Horse
Dog
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Elephant
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| WB |
1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 123 kDa).
|
|
| IP |
Use at 2-5 µg/mg of lysate.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 123 kDa). |
|
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. -
Tissue specificity
Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. -
Sequence similarities
Belongs to the SMARCC family.
Contains 1 SANT domain.
Contains 1 SWIRM domain. -
Post-translational
modificationsPhosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6599 Human
- Omim: 601732 Human
- SwissProt: Q92922 Human
- Unigene: 476179 Human
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Alternative names
- AI115498 antibody
- BAF 155 antibody
- BAF155 antibody
see all
Images
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Western blot - Anti-SMARCC1/BAF155 antibody (ab72503)All lanes : Anti-SMARCC1/BAF155 antibody (ab72503) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 123 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 1 second -
Immunoprecipitation - Anti-SMARCC1/BAF155 antibody (ab72503)Detection of SMARCC1/BAF155 by Western Blot of Immunprecipitate.
ab72503 at 1µg/ml staining SMARCC1/BAF155 in HeLa whole cell lysate immunoprecipitated using ab72503 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 1 second. -
Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody (ab72503)ICC/IF image of ab72503 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72503, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab72503 has been referenced in 1 publication.
- Chen L et al. Transcriptional control in cardiac progenitors: Tbx1 interacts with the BAF chromatin remodeling complex and regulates Wnt5a. PLoS Genet 8:e1002571 (2012). WB ; Mouse . PubMed: 22438823