Overview

  • Product name

    Anti-SMARCC1/BAF155 antibody [EPR12389]
    See all SMARCC1/BAF155 primary antibodies
  • Description

    Rabbit monoclonal [EPR12389] to SMARCC1/BAF155
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SMARCC1/BAF155 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q92922

  • Positive control

    • NTera2/D1, HeLa, HEK-293 and Jurkat whole cell lysate (ab7899). HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as SMARCC1.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
    • Tissue specificity

      Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.
    • Sequence similarities

      Belongs to the SMARCC family.
      Contains 1 SANT domain.
      Contains 1 SWIRM domain.
    • Post-translational
      modifications

      Phosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • AI115498 antibody
      • BAF 155 antibody
      • BAF155 antibody
      • BRG 1 associated factor 155 antibody
      • BRG1 associated factor 155 antibody
      • BRG1-associated factor 155 antibody
      • Chromatin remodeling complex BAF155 subunit antibody
      • CRACC 1 antibody
      • CRACC1 antibody
      • Mammalian chromatin remodeling complex BRG 1 associated factor 155 antibody
      • Mammalian chromatin remodeling complex BRG1 associated factor 155 antibody
      • Rsc 8 antibody
      • Rsc8 antibody
      • SMARC C1 antibody
      • SMARCC 1 antibody
      • SMARCC1 antibody
      • SMRC1_HUMAN antibody
      • SRG 3 antibody
      • SRG3 antibody
      • SWI 3 antibody
      • SWI/SNF complex 155 kDa subunit antibody
      • SWI/SNF complex subunit SMARCC1 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin c1 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 antibody
      • SWI3 antibody
      see all

    Images

    • All lanes : Anti-SMARCC1/BAF155 antibody [EPR12389] (ab172636) at 1/5000 dilution

      Lane 1 : Wild-type HEK-293 whole cell lysate
      Lane 2 : SMARCC1 knockout HEK-293 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : Jurkat whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 123 kDa
      Observed band size: 123 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab172636 observed at 123 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab172636 was shown to specifically react with SMARCC1 in wild-type HEK-293 cells as signal was lost in SMARCC1 knockout cells. Wild-type and SMARCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab172636 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling SMARCC1/BAF155 with ab172636 at a 1/100 dilution.

    • All lanes : Anti-SMARCC1/BAF155 antibody [EPR12389] (ab172636) at 1/1000 dilution

      Lane 1 : NTera2/D1 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 123 kDa

    References

    ab172636 has not yet been referenced specifically in any publications.

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