Product nameAnti-SMARCC1/BAF155 antibody [EPR12395] - ChIP Grade
See all SMARCC1/BAF155 primary antibodies
DescriptionRabbit monoclonal [EPR12395] to SMARCC1/BAF155 - ChIP Grade
Tested applicationsSuitable for: ChIP, WB, ICC/IF, IP, Flow Cytmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human SMARCC1/BAF155 aa 700-800 (Cysteine residue). The exact sequence is proprietary.
Database link: Q92922
- HeLa, K562, Jurkat and 293T cell lysates. Permeabilized Jurkat cells. Immunoprecipitation pellet from Jurkat whole cell lysate (ab7899). Rat testis lysates.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as SMARCC1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.21% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab172638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 123 kDa.|
For unpurified use at 1/250 - 1/500.
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Tissue specificityExpressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.
Sequence similaritiesBelongs to the SMARCC family.
Contains 1 SANT domain.
Contains 1 SWIRM domain.
modificationsPhosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein.
- Information by UniProt
- AI115498 antibody
- BAF 155 antibody
- BAF155 antibody
All lanes : Anti-SMARCC1/BAF155 antibody [EPR12395] - ChIP Grade (ab172638) at 1/5000 dilution
Lane 1 : Wild-type HEK293 whole cell lysate
Lane 2 : SMARCC1 knockout HEK293 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 123 kDa
Lanes 1 - 4: Merged signal (red and green). Green - ab172638 observed at 123 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab172638 was shown to recognize in wild-type HEK293 cells as signal was lost at the expected MW in SMARCC1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SMARCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab172638 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SMARCC2 with Purified ab172638 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling SMARCC1/BAF155 with purified ab172638 at 1:30 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Chromatin was prepared from MDA-MB-231 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab172638 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci).
Primers and probes are located in the first kb of the transcribed region.
All lanes : Anti-SMARCC1/BAF155 antibody [EPR12395] - ChIP Grade (ab172638) at 1/5000 dilution (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Rat testis lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 123 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer: 5% NFDM/TBST.
ab172638 (purified) at 1:30 dilution (2μg) immunoprecipitating SMARCC1/BAF155 in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, 10μg
Lane 2 (+): ab172638 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab172638 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST."
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using unpurified ab172638 at a 1/10 dilution.
Flow cytometric analysis of permeabilized Jurkat cells using unpurified ab172638 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
All lanes : Anti-SMARCC1/BAF155 antibody [EPR12395] - ChIP Grade (ab172638) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 123 kDa
ab172638 has been referenced in 3 publications.
- Wu S et al. ARID1A spatially partitions interphase chromosomes. Sci Adv 5:eaaw5294 (2019). PubMed: 31131328
- Scott CA et al. Nuclear/cytoplasmic transport defects in BBS6 underlie congenital heart disease through perturbation of a chromatin remodeling protein. PLoS Genet 13:e1006936 (2017). PubMed: 28753627
- Cabot B et al. Differential expression of key subunits of SWI/SNF chromatin remodeling complexes in porcine embryos derived in vitro or in vivo. Mol Reprod Dev 84:1238-1249 (2017). ICC/IF . PubMed: 29024220