Overview

  • Product name

  • Description

    Rabbit polyclonal to SMARCD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Proteus mirabilis, Orangutan, Medaka fish
  • Immunogen

    Synthetic peptide, corresponding to amino acids 425-475 of Human SMARCD2

  • Positive control

    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab81622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      SMARCD2 is a member of the SWI/SNF family of proteins which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. It is part of the large ATP-dependent chromatin remodeling complex SNF/SWI.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • 60 kDa BRG 1/Brm associated factor subunit B antibody
      • 60 kDa BRG1/Brm associated factor subunit B antibody
      • BAF60B antibody
      • BRG1 associated factor 60B antibody
      • Chromatin remodeling complex BAF60B subunit antibody
      • CRACD 2 antibody
      • CRACD2 antibody
      • Mammalian chromatin remodeling complex BRG1 associated factor 60B antibody
      • PRO2451 antibody
      • Rsc6p antibody
      • SMARCD 2 antibody
      • SWI/SNF complex 60 kDa subunit B antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin d2 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily d member 2 antibody
      • Swp73 like protein antibody
      see all

    Images

    • Anti-SMARCD2 antibody (ab81622) at 0.04 µl/ml buffer
      Developed using the ECL technique.

      Predicted band size: 52 kDa


      Exposure time: 30 seconds


      Whole cell lysate (50, 15 and 5 µg) from HeLa cells

    References

    This product has been referenced in:

    • Rhyasen GW  et al. BRD4 amplification facilitates an oncogenic gene expression program in high-grade serous ovarian cancer and confers sensitivity to BET inhibitors. PLoS One 13:e0200826 (2018). Read more (PubMed: 30036377) »
    • Goljanek-Whysall K  et al. myomiR-dependent switching of BAF60 variant incorporation into Brg1 chromatin remodeling complexes during embryo myogenesis. Development 141:3378-87 (2014). WB ; Mouse . Read more (PubMed: 25078649) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (mouse neural stem cell)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    mouse neural stem cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

    Dr. Robert Kupp

    Verified customer

    Submitted Sep 17 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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