
Recombinant Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-SMARCD2 antibody [EPR20860-251]
See all SMARCD2 primary antibodies -
Description
Rabbit monoclonal [EPR20860-251] to SMARCD2 -
Host species
Rabbit -
Specificity
IHC is recommended only for mouse and rat.
-
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SMARCD2 aa 300-400. The exact sequence is proprietary.
Database link: Q99JR8 -
Positive control
- WB: Neuro-2a cells transfected with scrambled siRNA control, whole cell lysate; mouse P70 thymus, white adipose, brown adipose and liver lysate; Beta-TC-6 whole cell lysate; Rat thymus lysate; Jurkat and HeLa whole cell lysate. IHC-P: Mouse colon tissue; Rat testis tissue. Flow cyt: HeLa cells. IP: HeLa whole cell lysate; Mouse P70 thymus lysate.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20860-251 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Immunohistochemistry kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab220164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). | |
IHC-P | 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
Flow Cyt | 1/600. | |
IP | 1/30. |
Target
-
Relevance
SMARCD2 is a member of the SWI/SNF family of proteins which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. It is part of the large ATP-dependent chromatin remodeling complex SNF/SWI. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 6603 Human
- Entrez Gene: 83796 Mouse
- Entrez Gene: 83833 Rat
- Omim: 601736 Human
- SwissProt: Q92925 Human
- SwissProt: Q99JR8 Mouse
- SwissProt: O54772 Rat
- Unigene: 250581 Human
see all -
Alternative names
- 60 kDa BRG 1/Brm associated factor subunit B antibody
- 60 kDa BRG1/Brm associated factor subunit B antibody
- BAF60B antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SMARCD2 with ab220164 at 1/200 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis (PMID: 20148946, 28303890, 25622893). The section was incubated with ab220164 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
SMARCD2 was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab220164 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220164 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab220164 IP in HeLa whole cell lysate .
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220164 in HeLa whole cell lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 40 seconds. -
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling SMARCD2 with ab220164 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling SMARCD2 with ab220164 at 1/200 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse colon (PMID: 20148946, 28303890, 25622893). The section was incubated with ab220164 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
SMARCD2 was immunoprecipitated from 0.35 mg mouse P70 thymus lysate with ab220164 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220164 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: Mouse P70 thymus lysate 10 µg (Input).
Lane 2: ab220164 IP in mouse P70 thymus lysate .
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220164 in mouse P70 thymus lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 seconds. -
All lanes : Anti-SMARCD2 antibody [EPR20860-251] (ab220164) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Rat thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 59 secs; Lane 2: 81 secs; Lane 3: 26 secs.
-
All lanes : Anti-SMARCD2 antibody [EPR20860-251] (ab220164) at 1/1000 dilution
Lane 1 : Mouse P70 liver lysate
Lane 2 : Mouse P70 thymus lysate
Lane 3 : Mouse P70 white adipose lysate
Lane 4 : Mouse P70 brown adipose lysate
Lane 5 : Beta-TC-6 (mouse pancreas insulinoma beta cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-SMARCD2 antibody [EPR20860-251] (ab220164) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) transfected with scrambled siRNA control whole cell lysate
Lane 2 : SMARCD2 knockdown Neuro-2a whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 3 minutesLysates were kindly provided by Dr Zhuoxian Meng.
Blocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
References
ab220164 has not yet been referenced specifically in any publications.