RecombinantRabMAb

Recombinant Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20860-251] to SMARCD2
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SMARCD2 antibody [EPR20860-251]
    See all SMARCD2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20860-251] to SMARCD2

  • Host species

    Rabbit

  • Specificity

    IHC is recommended only for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SMARCD2 aa 300-400. The exact sequence is proprietary.
    Database link: Q99JR8

  • Positive control

    • WB: Neuro-2a cells transfected with scrambled siRNA control, whole cell lysate; mouse P70 thymus, white adipose, brown adipose and liver lysate; Beta-TC-6 whole cell lysate; Rat thymus lysate; Jurkat and HeLa whole cell lysate. IHC-P: Mouse colon tissue; Rat testis tissue. Flow cyt: HeLa cells. IP: HeLa whole cell lysate; Mouse P70 thymus lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab220164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/600.
IP 1/30.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Relevance

      SMARCD2 is a member of the SWI/SNF family of proteins which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. It is part of the large ATP-dependent chromatin remodeling complex SNF/SWI.

    • Cellular localization

      Nuclear

    • Database links

      see all

    • Alternative names

      • 60 kDa BRG 1/Brm associated factor subunit B antibody
      • 60 kDa BRG1/Brm associated factor subunit B antibody
      • BAF60B antibody
      • BRG1 associated factor 60B antibody
      • Chromatin remodeling complex BAF60B subunit antibody
      • CRACD 2 antibody
      • CRACD2 antibody
      • Mammalian chromatin remodeling complex BRG1 associated factor 60B antibody
      • PRO2451 antibody
      • Rsc6p antibody
      • SMARCD 2 antibody
      • SWI/SNF complex 60 kDa subunit B antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin d2 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily d member 2 antibody
      • Swp73 like protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

      Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SMARCD2 with ab220164 at 1/200 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis (PMID: 20148946, 28303890, 25622893). The section was incubated with ab220164 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunoprecipitation - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
      Immunoprecipitation - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

      SMARCD2 was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab220164 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220164 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

      Lane 1: HeLa whole cell lysate 10 µg (Input).
      Lane 2: ab220164 IP in HeLa whole cell lysate .
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220164 in HeLa whole cell lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 40 seconds.

    • Flow Cytometry - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
      Flow Cytometry - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling SMARCD2 with ab220164 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).

      Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

      Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling SMARCD2 with ab220164 at 1/200 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse colon (PMID: 20148946, 28303890, 25622893). The section was incubated with ab220164 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunoprecipitation - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
      Immunoprecipitation - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

      SMARCD2 was immunoprecipitated from 0.35 mg mouse P70 thymus lysate with ab220164 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220164 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

      Lane 1: Mouse P70 thymus lysate 10 µg (Input).
      Lane 2: ab220164 IP in mouse P70 thymus lysate .
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220164 in mouse P70 thymus lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 3 seconds.

    • Western blot - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
      Western blot - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
      All lanes : Anti-SMARCD2 antibody [EPR20860-251] (ab220164) at 1/1000 dilution

      Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : Rat thymus lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 59 kDa
      Observed band size: 59 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1: 59 secs; Lane 2: 81 secs; Lane 3: 26 secs.

    • Western blot - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
      Western blot - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
      All lanes : Anti-SMARCD2 antibody [EPR20860-251] (ab220164) at 1/1000 dilution

      Lane 1 : Mouse P70 liver lysate
      Lane 2 : Mouse P70 thymus lysate
      Lane 3 : Mouse P70 white adipose lysate
      Lane 4 : Mouse P70 brown adipose lysate
      Lane 5 : Beta-TC-6 (mouse pancreas insulinoma beta cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 59 kDa
      Observed band size: 59 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
      Western blot - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
      All lanes : Anti-SMARCD2 antibody [EPR20860-251] (ab220164) at 1/1000 dilution

      Lane 1 : Neuro-2a (mouse neuroblastoma cell line) transfected with scrambled siRNA control whole cell lysate
      Lane 2 : SMARCD2 knockdown Neuro-2a whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 59 kDa
      Observed band size: 59 kDa


      Exposure time: 3 minutes


      Lysates were kindly provided by Dr Zhuoxian Meng.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-SMARCD2 antibody [EPR20860-251] (ab220164)
      Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab220164? Please let us know so that we can cite the reference in this datasheet.

    ab220164 has not yet been referenced specifically in any publications.

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