Overview

  • Product name
    Anti-SMC1 antibody - ChIP Grade
    See all SMC1 primary antibodies
  • Description
    Rabbit polyclonal to SMC1 - ChIP Grade
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC, IP, WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH - which represents a portion within the last 100 amino acids of the human Structural Maintenance of Chromosomes-1 (GenBank PID 5453642).

Properties

Applications

Our Abpromise guarantee covers the use of ab9262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/200 - 1/800.
IP Use at 1-4 µg/mg of lysate.
WB 1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
ChIP Use at an assay dependent concentration. PubMed: 20966046

Target

Images

  • All lanes : Anti-SMC1 antibody - ChIP Grade (ab9262) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 143 kDa


    Exposure time: 3 seconds


    Detection: Chemiluminescence.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SMC1 with ab9262 at 1/1000 (1µg/ml). Detection: DAB.
  • Interphase HeLa cells stained with ab9262 (1/500). The antibody gave nuclear staining in all interphase nuclei investigated. However,  the antibody failed to recognize any chromatin-associated epitopes in prophase or metaphase cells suggesting that the epitopes may be masked during mitosis. ab9262 staining is shown in green. The cells were counterstained with DAPI (red). 100x magnification.

  • Immunoprecipitation analysis of HeLa whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

    Lanes 1 & 2: ab9262 was used for IP at 6 µg per reaction.
    Lane 3: IP using rabbit anti-SMC1A antibody (ab140493).
    Lane 4: Control IgG.

    For western blotting immunoprecipitated SMC1, ab9262 was used at 1 µg/ml.
    Detection: Chemiluminescence.

  • Ab9262 staining human normal colon tissue. Staining is localised to nuclear compartment.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

This product has been referenced in:
  • Mehta K & Laimins L Human Papillomaviruses Preferentially Recruit DNA Repair Factors to Viral Genomes for Rapid Repair and Amplification. MBio 9:N/A (2018). Read more (PubMed: 29440569) »
  • Tripathi V  et al. MRN complex-dependent recruitment of ubiquitylated BLM helicase to DSBs negatively regulates DNA repair pathways. Nat Commun 9:1016 (2018). Read more (PubMed: 29523790) »
See all 25 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Non-reduced Denaturing (7.5)
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted May 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Loading amount
50 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MRC5VA Lung Epithelial Fibroblasts)
Loading amount
15 µg
Specification
MRC5VA Lung Epithelial Fibroblasts
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Apr 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
40 µg
Specification
HeLa cells
Treatment
X radiation (10g)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted May 02 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Dec 07 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - nuclear (Egg extract)
Specification
Egg extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5

Dr. Alan Score

Verified customer

Submitted Sep 21 2005

Answer

Thank you for your interest in this antibody. It has only been tested for reactivity to the human ortholog. The 16 aa peptide used to generate and affinity purify this antibody is 100% identical to sequences found in human, mouse, rat, and cow SMC1. Fourteen continuous residues are identical to that found in Xenopus. One of the 2 mismatches with Xenopus is a conserved substitution (D vs E). If this is information is not sufficient. Please send the accession number or last 100 residues of SMC1 in the species of your interest, and I will let you know the degree of identity and homology with your ortholog

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Question
Answer

No research has been published yet using this antibody. We welcome feedback from researchers using our antibodies and can provide an Amazon voucher for antibody reviews.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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