Anti-SMC1A (phospho S966) antibody (ab1276)
Key features and details
- Rabbit polyclonal to SMC1A (phospho S966)
- Suitable for: ICC, IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SMC1A (phospho S966) antibody
See all SMC1A primary antibodies -
Description
Rabbit polyclonal to SMC1A (phospho S966) -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide within SMC1A (phospho S966) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q14683 -
Positive control
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate. ICC: HeLa and SKN cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antibodies that were not phospho-specific were removed by solid phase absorption. Antibodies specific for SMC1A pSer957 were affinity purified using the phosphopeptide immobilized onsolid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC | (1) |
1/500 - 1/5000.
|
IP |
Use at 2-5 µg/mg of lysate.
|
|
WB | (1) |
1/500 - 1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
|
Notes |
---|
ICC
1/500 - 1/5000. |
IP
Use at 2-5 µg/mg of lysate. |
WB
1/500 - 1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa). |
Target
-
Function
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint. -
Involvement in disease
Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies. -
Sequence similarities
Belongs to the SMC family. SMC1 subfamily. -
Domain
The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure. -
Post-translational
modificationsPhosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere > kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis. - Information by UniProt
-
Database links
- Entrez Gene: 8243 Human
- Omim: 300040 Human
- SwissProt: Q14683 Human
- Unigene: 211602 Human
-
Alternative names
- Chromosome segregation protein SmcB antibody
- DXS423E antibody
- KIAA0178 antibody
see all
Images
-
All lanes : Anti-SMC1A (phospho S966) antibody (ab1276) at 0.1 µg/ml
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with 100 µM etoposide (+)
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with 100 µM etoposide (-)
Lysates/proteins at 50 µg per lane.
Predicted band size: 143 kDa
Exposure time: 3 minutes -
SMC1A was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate treated with 100 µM etoposide (+) or mock treated (-) with ab1276 at 1 µg/ml. Western blot was performed from the immunoprecipitate using ab1276 at 1/1000 dilution.
-
Immunocytochemistry analysis of neocarzinostatin treated asynchronous HeLa cells labelling SMC1A (phospho S966) with ab1276 at 1/5000 (0.2µg/ml). A DyLight® 594-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.
-
ICC of SKN cells cultured on coverslips were fixed in 4% paraformaldehyde and then stained with Rabbit polyclonal to SMC1A (phospho S966), ab1276 (green) at a working dilution of 1/200. The DNA stained with DAPI is shown in red. (100x magnification).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (17)
ab1276 has been referenced in 17 publications.
- Yi F et al. The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis. Sci Adv 7:N/A (2021). PubMed: 33627431
- Leimbacher PA et al. MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis. Mol Cell 74:571-583.e8 (2019). PubMed: 30898438
- Zhang Y et al. Phosphorylation of SMC1A promotes hepatocellular carcinoma cell proliferation and migration. Int J Biol Sci 14:1081-1089 (2018). PubMed: 29988990
- Yang T et al. Quercetin-3-O-ß-D-glucoside decreases the bioavailability of cyclosporin A through regulation of drug metabolizing enzymes, transporters and nuclear receptors in rats. Mol Med Rep 18:2599-2612 (2018). PubMed: 30015887
- Jiang Y et al. ATM function and its relationship with ATM gene mutations in chronic lymphocytic leukemia with the recurrent deletion (11q22.3-23.2). Blood Cancer J 6:e465 (2016). PubMed: 27588518