Overview

  • Product name

  • Description

    Goat polyclonal to SMC1A
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat, Platypus
  • Immunogen

    Synthetic peptide corresponding to Human SMC1A aa 1183-1233.
    Sequence:

    QAIVISLK EEFYTKAESL IGVYPEQGDC VISKVLTFDL TKYPDANPNP NEQ

  • Positive control

    • 293T, HeLa, Jurkat whole cell lysate (ab7899); NIH3T3 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab140493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
  • Involvement in disease

    Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
  • Sequence similarities

    Belongs to the SMC family. SMC1 subfamily.
  • Domain

    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
  • Post-translational
    modifications

    Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere > kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chromosome segregation protein SmcB antibody
    • DXS423E antibody
    • KIAA0178 antibody
    • MGC138332 antibody
    • Sb1.8 antibody
    • Segregation of mitotic chromosomes 1 antibody
    • SMC protein 1A antibody
    • SMC-1-alpha antibody
    • SMC-1A antibody
    • SMC1 (structural maintenance of chromosomes 1 yeast) like 1 antibody
    • SMC1 antibody
    • SMC1 structural maintenance of chromosomes 1 like 1 antibody
    • SMC1A antibody
    • SMC1A_HUMAN antibody
    • SMC1alpha antibody
    • SMC1L1 antibody
    • SMCB antibody
    • Structural maintenance of chromosomes 1A antibody
    • Structural maintenance of chromosomes protein 1A antibody
    see all

Images

  • All lanes : Anti-SMC1A antibody (ab140493) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 143 kDa


    Exposure time: 3 minutes
  • All lanes : Anti-SMC1A antibody (ab140493) at 0.4 µg/ml

    Lane 1 : NIH3T3 whole cell lysate at 50 µg
    Lane 2 : NIH3T3 whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 143 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation of SMC1A from Hela whole cell lysate by ab140493 at 6 µg/mg lysate (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control. Detection: chemiluminescence. Exposure time: 30 seconds.

References

ab140493 has not yet been referenced specifically in any publications.

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