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  1. Link

    smc1a-antibody-ab9262.pdf

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Cell Biology Cell Cycle Cell Division Chromatid Cohesion
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Anti-SMC1A antibody (ab9262)

  • Datasheet
  • SDS
Reviews (6)Q&A (4)References (37)

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Western blot - Anti-SMC1A antibody (ab9262)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)
  • Immunoprecipitation - Anti-SMC1A antibody (ab9262)

Key features and details

  • Rabbit polyclonal to SMC1A
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-SMC1A antibody [BL-205-2G8] (ab243875)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-SMC1A antibody
    See all SMC1A primary antibodies
  • Description

    Rabbit polyclonal to SMC1A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide within Human SMC1A aa 1133-1233 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q14683

  • Positive control

    • WB: HeLa, 293T and NIH3T3 whole cell lysate. IHC-P: Human ovarian cancer tissue, mouse colon cancer tissue CT26. IP: HeLa whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280nm of 1.4 equals 1.0 mg of IgG.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Cohesins
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

Associated products

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at 1-4 µg/mg of lysate.
WB (5)
1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
Notes
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at 1-4 µg/mg of lysate.
WB
1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).

Target

  • Function

    Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
  • Involvement in disease

    Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
  • Sequence similarities

    Belongs to the SMC family. SMC1 subfamily.
  • Domain

    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
  • Post-translational
    modifications

    Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere > kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.
  • Target information above from: UniProt accession Q14683 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282370 Cow
    • Entrez Gene: 8243 Human
    • Entrez Gene: 24061 Mouse
    • Entrez Gene: 63996 Rat
    • Omim: 300040 Human
    • SwissProt: O97593 Cow
    • SwissProt: Q14683 Human
    • SwissProt: Q9CU62 Mouse
    • SwissProt: Q9Z1M9 Rat
    • Unigene: 211602 Human
    see all
  • Alternative names

    • Chromosome segregation protein SmcB antibody
    • DXS423E antibody
    • KIAA0178 antibody
    • MGC138332 antibody
    • Sb1.8 antibody
    • Segregation of mitotic chromosomes 1 antibody
    • SMC protein 1A antibody
    • SMC-1-alpha antibody
    • SMC-1A antibody
    • SMC1 (structural maintenance of chromosomes 1 yeast) like 1 antibody
    • SMC1 antibody
    • SMC1 structural maintenance of chromosomes 1 like 1 antibody
    • SMC1A antibody
    • SMC1A_HUMAN antibody
    • SMC1alpha antibody
    • SMC1L1 antibody
    • SMCB antibody
    • Structural maintenance of chromosomes 1A antibody
    • Structural maintenance of chromosomes protein 1A antibody
    see all

Images

  • Western blot - Anti-SMC1A antibody (ab9262)
    Western blot - Anti-SMC1A antibody (ab9262)
    All lanes : Anti-SMC1A antibody (ab9262) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 143 kDa


    Exposure time: 3 seconds


    Detection: Chemiluminescence.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian cancer tissue, labeling SMC1 with ab9262 at 0.2 µg/mL. Detection: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse colon cancer tissue CT26, labeling SMC1 with ab9262 at 0.2 µg/mL. Detection: DAB.

  • Immunoprecipitation - Anti-SMC1A antibody (ab9262)
    Immunoprecipitation - Anti-SMC1A antibody (ab9262)

    SMC1A was immunoprecipitated from 1 mg HeLa whole cell lysate with ab9262 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab243875 at 1/1000 dilution.

    Lysates prepared using NETN lysis buffer.

    Lane 1: ab9262 IP in HeLa whole cell lysate.

    Lane 2: Contol IgG in HeLa whole cell lysate.

    Detection: Chemiluminescence.

    Exposure time: 10 seconds.

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (37)

Publishing research using ab9262? Please let us know so that we can cite the reference in this datasheet.

ab9262 has been referenced in 37 publications.

  • Li X  et al. Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes. Virol J 18:26 (2021). PubMed: 33485391
  • Bailey ML  et al. Paralogous synthetic lethality underlies genetic dependencies of the cancer-mutated gene STAG2. Life Sci Alliance 4:N/A (2021). PubMed: 34462321
  • Chai P  et al. Generation of onco-enhancer enhances chromosomal remodeling and accelerates tumorigenesis. Nucleic Acids Res 48:12135-12150 (2020). PubMed: 33196849
  • Jia L  et al. Oplr16 serves as a novel chromatin factor to control stem cell fate by modulating pluripotency-specific chromosomal looping and TET2-mediated DNA demethylation. Nucleic Acids Res 48:3935-3948 (2020). PubMed: 32055844
  • Reichmann J  et al. Tex19.1 inhibits the N-end rule pathway and maintains acetylated SMC3 cohesin and sister chromatid cohesion in oocytes. J Cell Biol 219:N/A (2020). PubMed: 32232464
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 10 Abreviews or Q&A

Western blot

Good
Abreviews
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Non-reduced Denaturing (7.5)
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted May 28 2013

Western blot abreview for Anti-SMC1 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Loading amount
50 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Aug 01 2012

Western blot abreview for Anti-SMC1 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MRC5VA Lung Epithelial Fibroblasts)
Loading amount
15 µg
Specification
MRC5VA Lung Epithelial Fibroblasts
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Apr 13 2011

Western blot abreview for Anti-SMC1 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
40 µg
Specification
HeLa cells
Treatment
X radiation (10g)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Read More

Abcam user community

Verified customer

Submitted May 02 2007

Immunocytochemistry abreview for Anti-SMC1 antibody - ChIP Grade

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Read More

DR. Kirk Mcmanus

Verified customer

Submitted Dec 07 2005

Western blot abreview for Anti-SMC1 antibody - ChIP Grade

Good
Abreviews
Abreviews
Application
Western blot
Sample
Xenopus laevis Cell lysate - nuclear (Egg extract)
Specification
Egg extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Read More

DR. Alan Score

Verified customer

Submitted Sep 21 2005

Question

Bonjour, J'ai reçu très récessement ma commande (voir les numéros en objet) d'anticorps ab9262 (SMC1 rabbit polyclonal). Lorsque je l'ai aliquoté, je me suis apperçu qu'il y avait à peine 40µL dans le tube. Je pense donc que je n'ai pas les 50µg d'anticorps prévu. Je voudrais donc savoir ce qu'il faut faire ou ce que vous faites dans ces cas là. I recently received an order (see the number in the subject of this email) of antibody ab9262 (SMC1 rabbit polyclonal). I aliquoted and observed that there were barely 40 microliters in the tube. I do not think that I received the 50 micrograms antibody which I expected. I was wondering what I should do or what your policy is in such cases.

Read More

Abcam community

Verified customer

Asked on Sep 16 2005

Answer

Thank you for your enquiry and I apologize for the shortage that you received in your vial of ab9262. I have arranged for a free of charge replacement vial to be sent to you. It is order reference # 102521 and you will receive it this week. If you have any additional questions or concerns, please do let us know.

Read More

Abcam Scientific Support

Answered on Sep 20 2005

Question

Recently our lab ordered abcam ab 9262-50 SMC-1 rabbit polyclonal to human antibody lot # 11779. The order was placed under the name Paige Pardington. Upon receipt, the tube was approximately half of the expected volume (22ul). After centrifuging the vial prior to opening and taking two aliquots of 3ul each, closing the vial and returning the vial to the 4 degree there is no volume left at all after two weeks. There appears to be a residue left on the inside of the tube. Are you having problems with filling the vials or with the seal of the vials?

Read More

Abcam community

Verified customer

Asked on Apr 30 2003

Answer

We have not received similar reports from other customers, so I think it is likely that the seal on the vial you received is faulty. We will send you a replacement vial tomorrow.

Read More

Abcam Scientific Support

Answered on Apr 30 2003

Question

Can you tell me specifically which amino acids were used to raise the SMC1 antibody? I would want to use it to detect SMC1 from another species.

Read More

Abcam community

Verified customer

Asked on Sep 10 2002

Answer

Thank you for your interest in this antibody. It has only been tested for reactivity to the human ortholog. The 16 aa peptide used to generate and affinity purify this antibody is 100% identical to sequences found in human, mouse, rat, and cow SMC1. Fourteen continuous residues are identical to that found in Xenopus. One of the 2 mismatches with Xenopus is a conserved substitution (D vs E). If this is information is not sufficient. Please send the accession number or last 100 residues of SMC1 in the species of your interest, and I will let you know the degree of identity and homology with your ortholog

Read More

Abcam Scientific Support

Answered on Sep 17 2002

Question

Could you supply a reference that applied your anti-human SMC1 antibody in their research?

Read More

Abcam community

Verified customer

Asked on May 22 2002

Answer

No research has been published yet using this antibody. We welcome feedback from researchers using our antibodies and can provide an Amazon voucher for antibody reviews.

Read More

Abcam Scientific Support

Answered on May 23 2002

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