Overview

  • Product name

    Anti-SMC1A antibody [EPFHCR37F]
    See all SMC1A primary antibodies
  • Description

    Rabbit monoclonal [EPFHCR37F] to SMC1A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SMC1A aa 1200-1300 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab209494)

  • Positive control

    • HeLa, 293T, Jurkat and Saos-2 cell lysates; Human colon tissue; Saos-2 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPFHCR37F
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 143 kDa.Can be blocked with SMC1A peptide (ab209494).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
    • Involvement in disease

      Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
    • Sequence similarities

      Belongs to the SMC family. SMC1 subfamily.
    • Domain

      The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
    • Post-translational
      modifications

      Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.
    • Cellular localization

      Nucleus. Chromosome. Chromosome > centromere > kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.
    • Information by UniProt
    • Database links

    • Alternative names

      • Chromosome segregation protein SmcB antibody
      • DXS423E antibody
      • KIAA0178 antibody
      • MGC138332 antibody
      • Sb1.8 antibody
      • Segregation of mitotic chromosomes 1 antibody
      • SMC protein 1A antibody
      • SMC-1-alpha antibody
      • SMC-1A antibody
      • SMC1 (structural maintenance of chromosomes 1 yeast) like 1 antibody
      • SMC1 antibody
      • SMC1 structural maintenance of chromosomes 1 like 1 antibody
      • SMC1A antibody
      • SMC1A_HUMAN antibody
      • SMC1alpha antibody
      • SMC1L1 antibody
      • SMCB antibody
      • Structural maintenance of chromosomes 1A antibody
      • Structural maintenance of chromosomes protein 1A antibody
      see all

    Images

    • All lanes : Anti-SMC1A antibody [EPFHCR37F] (ab133643) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Saos-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 143 kDa

    • Immunofluorescent analysis of Saos-2 cells labelling SMC1A with ab133643 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling SMC1A with ab133643 at 1/100 dilution.

    References

    This product has been referenced in:

    • Shah VJ & Maddika S CRL7SMU1 E3 ligase complex-driven H2B ubiquitylation functions in sister chromatid cohesion by regulating SMC1 expression. J Cell Sci 131:N/A (2018). Read more (PubMed: 29507117) »
    • Li Z  et al. ASXL1 interacts with the cohesin complex to maintain chromatid separation and gene expression for normal hematopoiesis. Sci Adv 3:e1601602 (2017). Read more (PubMed: 28116354) »
    See all 4 Publications for this product

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