Product nameAnti-SMC1A (phospho S957) antibody
See all SMC1A primary antibodies
DescriptionRabbit polyclonal to SMC1A (phospho S957)
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Synthetic peptide within Human SMC1A (phospho S957). The exact sequence is proprietary. (NP_006297.2).
Database link: Q14683
- WB: Etoposide treated HEK-293T whole cell lysate. IP: Etoposide treated HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue; Mouse teratoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab240573 was affinity purified using an epitope specific to SMC1A (phospho S957) immobilized on solid support.
Our Abpromise guarantee covers the use of ab240573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 143 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Involvement in diseaseDefects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
Sequence similaritiesBelongs to the SMC family. SMC1 subfamily.
DomainThe flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
modificationsPhosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.
Cellular localizationNucleus. Chromosome. Chromosome > centromere > kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.
- Information by UniProt
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All lanes : Anti-SMC1A (phospho S957) antibody (ab240573) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (Mock treated) whole cell lysate
Lane 2 : HEK-293T, Treated with 100 uM etoposide for 2 hours, whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 143 kDa
Exposure time: 30 seconds
Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for SMC1A (phospho S957) with ab240573 at 1 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for (phospho S957) with ab240573 at 0.2 µg/ml in immunohistochemical analysis. DAB staining.
Detection of SMC1A (phospho S957) by IP.
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP; 20% of IP loaded) treated with 100 µM etopside for 2 hours (Lane 2) or mock treated (Lane 1).
ab240573 used at 6 µg/mg lysate and at 0.1 µg/ml for WB.
Lane 3: Control IgG IP in HEK-293T whole cell lysate mock treated.
Lane 4: Control IgG IP in HEK-293T whole cell lysate treated with 100 µM etopside for 2 hours.
Detection: Chemiluminescence with exposure times of 30 seconds.
ab240573 has not yet been referenced specifically in any publications.