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Cell Biology Cell Cycle Cell Division Chromatid Cohesion
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RabMAb

Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)

  • Datasheet
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Western blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)
  • Immunocytochemistry/ Immunofluorescence - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)

Key features and details

  • Rabbit monoclonal [EP2858Y] to SMC1A (phospho S966)
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-SMC1A (phospho S966) antibody [EP2858Y]
    See all SMC1A primary antibodies
  • Description

    Rabbit monoclonal [EP2858Y] to SMC1A (phospho S966)
  • Host species

    Rabbit
  • Specificity

    This antibody detects SMC1A phosphorylated on Serine 966.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    corresponding to Human SMC1A.

  • Positive control

    • HeLa whole cell lysate (ab150035). HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP2858Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Cohesins
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab81306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
Application Abreviews Notes
WB
1/5000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
ICC/IF
1/250.
Notes
WB
1/5000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
ICC/IF
1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
    • Involvement in disease

      Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
    • Sequence similarities

      Belongs to the SMC family. SMC1 subfamily.
    • Domain

      The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
    • Post-translational
      modifications

      Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.
    • Cellular localization

      Nucleus. Chromosome. Chromosome > centromere > kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.
    • Target information above from: UniProt accession Q14683 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8243 Human
      • Omim: 300040 Human
      • SwissProt: Q14683 Human
      • Unigene: 211602 Human
      • Alternative names

        • Chromosome segregation protein SmcB antibody
        • DXS423E antibody
        • KIAA0178 antibody
        • MGC138332 antibody
        • Sb1.8 antibody
        • Segregation of mitotic chromosomes 1 antibody
        • SMC protein 1A antibody
        • SMC-1-alpha antibody
        • SMC-1A antibody
        • SMC1 (structural maintenance of chromosomes 1 yeast) like 1 antibody
        • SMC1 antibody
        • SMC1 structural maintenance of chromosomes 1 like 1 antibody
        • SMC1A antibody
        • SMC1A_HUMAN antibody
        • SMC1alpha antibody
        • SMC1L1 antibody
        • SMCB antibody
        • Structural maintenance of chromosomes 1A antibody
        • Structural maintenance of chromosomes protein 1A antibody
        see all

      Images

      • Western blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)
        Western blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)
        All lanes : Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306) at 1/1000 dilution

        Lane 1 : HeLa cell lysates un-treated
        Lane 2 : HeLa cell lysates treated with hydroxyurea

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP goat anti-rabbit at 1/2000 dilution

        Predicted band size: 143 kDa
        Observed band size: 160 kDa why is the actual band size different from the predicted?

      • Immunocytochemistry/ Immunofluorescence - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)
        Immunocytochemistry/ Immunofluorescence - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)
        Immunofluorescent staining of HeLa cells using ab81306 at a 1/250 dilution.

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab81306? Please let us know so that we can cite the reference in this datasheet.

      ab81306 has not yet been referenced specifically in any publications.

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