Anti-SMC2 antibody (ab10412)
- Datasheet
- References (15)
- Protocols
Overview
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Product name
Anti-SMC2 antibody
See all SMC2 primary antibodies -
Description
Rabbit polyclonal to SMC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide. The epitope maps to a region between residue 1150 and the C-terminus (residue 1197) of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
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Positive control
- Whole cell lysate from mock transfected or SMC2 transfected HEK293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab10412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Detects a band of approximately 140 kDa (predicted molecular weight: 137 kDa). | |
| IP | Use at 2-10 µg/mg of lysate. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/200 - 1/400. |
Target
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Function
Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. -
Sequence similarities
Belongs to the SMC family. SMC2 subfamily. -
Domain
The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer. -
Cellular localization
Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. - Information by UniProt
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Database links
- Entrez Gene: 10592 Human
- Omim: 605576 Human
- SwissProt: O95347 Human
- Unigene: 119023 Human
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Alternative names
- CAP E antibody
- CAPE antibody
- Chromosome associated protein E antibody
see all
Images
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Western blot - Anti-SMC2 antibody (ab10412)ab10412 detection of human SMC2 by Western blot and Immunoprecipitation. Samples: Whole cell lysate (90
ab10412 detection of human SMC2 by Western blot and Immunoprecipitation. Samples: Whole cell lysate (90 µg for WB; 0.5 mg for IP) from HEK293T cells that were mock transfected (E), transfected with full length SMC2 (T for WB), or transfected with truncated SMC2 (T for IP). Antibody: ab10412 used at 0.1 µg/ml for WB and at 5 µg for IP. Detection: Chemiluminescence.µ g for WB; 0.5 mg for IP) from HEK293T cells that were mock transfected (E), transfected with full length SMC2 (T for WB), or transfected with truncated SMC2 (T for IP). Antibody: ab10412 used at 0.1µ g/ml for WB and at 5µ g for IP. Detection: Chemiluminescence. -
Immunocytochemistry/ Immunofluorescence - Anti-SMC2 antibody (ab10412)This image is courtesy of an Abreview submitted by Dr Kirk McManusab10412 (1/1000) staining SMC2 in assynchronous HeLa cells in conjunction with a secondary goat anti-rabbit antibody (1/200) conjugated to Cy3 ® (green). Cells were counterstained with DAPI to show the nucleus (red). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC2 antibody (ab10412)IHC image of ab10412 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10412, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC2 antibody (ab10412)Image from Dávalos V et al., The Journal of Biological Chemistry, 287, 43472-43481. December 21, 2012. Fig 1.; doi: 10.1074/jbc.M112.428466. Epub 2012 Oct 24.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colorectal tissue, staining SMC2 with ab10412 at 1/200 dilution. Staining was detected using DAB.
N, normal tissue; T: tumor tissue (adenocarcinoma)
Protocols
References
This product has been referenced in:
- Yang Y et al. NudCL2 is an Hsp90 cochaperone to regulate sister chromatid cohesion by stabilizing cohesin subunits. Cell Mol Life Sci 76:381-395 (2019). Read more (PubMed: 30368549) »
- Champion L et al. Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosis. Mol Biol Cell 30:427-440 (2019). Read more (PubMed: 30586323) »
