Key features and details
- Rabbit polyclonal to SMC2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SMC2 antibody
See all SMC2 primary antibodies
DescriptionRabbit polyclonal to SMC2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human SMC2 (internal sequence). The exact sequence is proprietary.
Database link: O95347
- IP: HeLa whole cell extract. WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 136 kDa.|
|IP||1/100 - 1/500.|
FunctionCentral component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Sequence similaritiesBelongs to the SMC family. SMC2 subfamily.
DomainThe hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer.
Cellular localizationNucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
- Information by UniProt
- CAP E antibody
- CAPE antibody
- Chromosome associated protein E antibody
All lanes : Anti-SMC2 antibody (ab229353) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell extracts
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 136 kDa
SMC2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab229353. Western blot was performed from the immunoprecipitate using ab229353.
Lane 1: HeLa whole cell extract.
Lane 2: Control IgG IP in HeLa whole cell extract.
Lane 3: ab229353 IP in HeLa whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229353 has not yet been referenced specifically in any publications.