Overview

  • Product name

  • Description

    Rabbit polyclonal to SMC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human SMC2 (internal sequence). The exact sequence is proprietary.
    Database link: O95347

  • Positive control

    • IP: HeLa whole cell extract. WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab229353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 136 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • Sequence similarities

    Belongs to the SMC family. SMC2 subfamily.
  • Domain

    The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer.
  • Cellular localization

    Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAP E antibody
    • CAPE antibody
    • Chromosome associated protein E antibody
    • Chromosome-associated protein E antibody
    • FLJ10093 antibody
    • hCAP E antibody
    • hCAP-E antibody
    • hCAPE antibody
    • PRO0324 antibody
    • SMC 2 antibody
    • SMC protein 2 antibody
    • SMC-2 antibody
    • SMC2 antibody
    • SMC2 L1 antibody
    • SMC2_HUMAN antibody
    • SMC2L1 antibody
    • Structural maintenance of chromosomes (SMC) family member chromosome associated protein E antibody
    • Structural maintenance of chromosomes 2 antibody
    • Structural maintenance of chromosomes 2 like 1 antibody
    • Structural maintenance of chromosomes 2 yeast like 1 antibody
    • Structural maintenance of chromosomes protein 2 antibody
    • XCAP E homolog antibody
    • XCAP-E homolog antibody
    • XCAPE homolog antibody
    see all

Images

  • All lanes : Anti-SMC2 antibody (ab229353) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell extracts
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 136 kDa



    5% SDS-PAGE

  • SMC2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab229353. Western blot was performed from the immunoprecipitate using ab229353.

    Lane 1: HeLa whole cell extract.

    Lane 2: Control IgG IP in HeLa whole cell extract.

    Lane 3: ab229353 IP in HeLa whole cell extract.

References

ab229353 has not yet been referenced specifically in any publications.

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