Key features and details
- Rabbit polyclonal to SMC3
- Suitable for: IHC-P, WB, IHC - Wholemount
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-SMC3 antibody
See all SMC3 primary antibodies
DescriptionRabbit polyclonal to SMC3
Tested applicationsSuitable for: IHC-P, WB, IHC - Wholemountmore details
Species reactivityReacts with: Zebrafish
Recombinant fragment within Zebrafish SMC3 aa 36-316. The exact sequence is proprietary.
Database link: 324475
- Zebrafish tissue and zebrafish whole cell lysate.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab210088 in the following tested applications.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 142 kDa.|
|IHC - Wholemount||1/100 - 1/500.|
FunctionCentral component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
Involvement in diseaseDefects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
Sequence similaritiesBelongs to the SMC family. SMC3 subfamily.
DomainThe flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
- Information by UniProt
- Entrez Gene: 324475 Zebrafish
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Anti-SMC3 antibody (ab210088) at 1/1000 dilution + zebrafish eye whole cell lysate at 30 µg
Predicted band size: 142 kDa
5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, labeling SMC3 using ab210088 at a 1/300 dilution.
Immunohistochemical analysis of whole-mount zebrafish embryo, labeling SMC3 using ab210088 at a 1/200 dilution.
ab210088 has not yet been referenced specifically in any publications.