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  1. Link

    smc3-antibody-ab9263.pdf

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Cell Biology Cell Cycle Cell Division Chromatid Cohesion
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Anti-SMC3 antibody (ab9263)

  • Datasheet
  • SDS
Reviews (7)Q&A (3)References (70)

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Western blot - Anti-SMC3 antibody (ab9263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (ab9263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (ab9263)
  • Immunoprecipitation - Anti-SMC3 antibody (ab9263)

Key features and details

  • Rabbit polyclonal to SMC3
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-SMC3 antibody [EPR7984] (ab128919)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-SMC3 antibody
    See all SMC3 primary antibodies
  • Description

    Rabbit polyclonal to SMC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to Human SMC3 conjugated to keyhole limpet haemocyanin. Which represents a portion within the last 100 amino acids GenBank PID 4885399.

  • Positive control

    • WB: HEK293T, HeLa, Jurkat, LNCaP, U2OS, A-549, TCMK-1, and NIH/3T3 whole cell lysate; IHC-P: Human breast carcinoma and mouse renal cell carcinoma; IP: Lysate from HEK293T cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • BRCA1
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Cohesins
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion
    • Cancer
    • Cell cycle
    • Cell division

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP (1)
Use at 2-10 µg/mg of lysate.
WB (3)
1/10000 - 1/25000. Detects a band of approximately 140 kDa (predicted molecular weight: 141.5 kDa).
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IP
Use at 2-10 µg/mg of lysate.
WB
1/10000 - 1/25000. Detects a band of approximately 140 kDa (predicted molecular weight: 141.5 kDa).
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
  • Involvement in disease

    Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
  • Sequence similarities

    Belongs to the SMC family. SMC3 subfamily.
  • Domain

    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
  • Target information above from: UniProt accession Q9UQE7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 395188 Chicken
    • Entrez Gene: 281729 Cow
    • Entrez Gene: 9126 Human
    • Entrez Gene: 13006 Mouse
    • Entrez Gene: 29486 Rat
    • Omim: 606062 Human
    • SwissProt: O97594 Cow
    • SwissProt: Q9UQE7 Human
    • SwissProt: Q9CW03 Mouse
    • SwissProt: Q5R4K5 Orangutan
    • SwissProt: P97690 Rat
    • Unigene: 24485 Human
    • Unigene: 14910 Mouse
    • Unigene: 11074 Rat
    see all
  • Alternative names

    • BAM antibody
    • Bamacan antibody
    • Basement membrane associated chondroitin proteoglycan antibody
    • Basement membrane-associated chondroitin proteoglycan antibody
    • BMH antibody
    • CDLS3 antibody
    • chondroitin sulfate proteoglycan 6 (bamacan) antibody
    • Chondroitin sulfate proteoglycan 6 antibody
    • Chromosome associated polypeptide antibody
    • Chromosome-associated polypeptide antibody
    • CSPG 6 antibody
    • CSPG6 antibody
    • hCAP antibody
    • Human chromosome associated polypeptide antibody
    • im:7142991 antibody
    • SMC 3 antibody
    • SMC protein 3 antibody
    • SMC-3 antibody
    • smc3 antibody
    • SMC3_HUMAN antibody
    • SMC3L1 antibody
    • Structural maintenance of chromosome 3 antibody
    • Structural maintenance of chromosomes 3 antibody
    • Structural maintenance of chromosomes protein 3 antibody
    • u:fb22e01 antibody
    • wu:fc30d07 antibody
    see all

Images

  • Western blot - Anti-SMC3 antibody (ab9263)
    Western blot - Anti-SMC3 antibody (ab9263)
    All lanes : Anti-SMC3 antibody (ab9263) at 0.04 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : LNCaP (human prostate cancer cell line) whole cell lysate
    Lane 5 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
    Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 7 : TCMK-1 (mouse epithelial cell line from kidney transformed with SV40 viral antigen) whole cell lysate
    Lane 8 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 141.5 kDa


    Exposure time: 3 seconds


    Lysates prepared using NETN lysis buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (ab9263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (ab9263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling SMC3 with ab9263 at 1/5000 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (ab9263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (ab9263)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labeling SMC3 with ab9263 at 1/5000 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.

  • Immunoprecipitation - Anti-SMC3 antibody (ab9263)
    Immunoprecipitation - Anti-SMC3 antibody (ab9263)

    Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.

    Lane 1: ab9263 used for IP at 6 µg per reaction

    Lane 2: Control IgG

    For blotting immunoprecipitated SMC3, ab9263 was used at 0.04 µg/mL.

    Detection: Chemiluminescence with an exposure time of 3 seconds.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (70)

Publishing research using ab9263? Please let us know so that we can cite the reference in this datasheet.

ab9263 has been referenced in 70 publications.

  • Li X  et al. Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes. Virol J 18:26 (2021). PubMed: 33485391
  • Carico ZM  et al. A cohesin cancer mutation reveals a role for the hinge domain in genome organization and gene expression. PLoS Genet 17:e1009435 (2021). PubMed: 33760811
  • Tian H  et al. EWSR1 affects PRDM9-dependent histone 3 methylation and provides a link between recombination hotspots and the chromosome axis protein REC8. Mol Biol Cell 32:1-14 (2021). PubMed: 33175657
  • Shiimori M  et al. Suv4-20h2 protects against influenza virus infection by suppression of chromatin loop formation. iScience 24:102660 (2021). PubMed: 34169237
  • Golov AK  et al. Sensitivity of cohesin-chromatin association to high-salt treatment corroborates non-topological mode of loop extrusion. Epigenetics Chromatin 14:36 (2021). PubMed: 34321070
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 10 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-SMC3 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Tachyglossus aculeatus Cell (Testicular cell suspension in 10% DMSO)
Permeabilization
Yes - 0.5% Triton X-100
Specification
Testicular cell suspension in 10% DMSO
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Filip Pajpach

Verified customer

Submitted Jun 04 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-SMC3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Scorpion Cell (spermatocyte at in pachytene)
Permeabilization
No
Specification
spermatocyte at in pachytene
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Bruno Almeida

Verified customer

Submitted Apr 27 2017

Western blot abreview for Anti-SMC3 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Loading amount
50 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 01 2012

Immunoprecipitation abreview for Anti-SMC3 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cytosolic and nuclear extracts)
Total protein in input
500 µg
Specification
HeLa cytosolic and nuclear extracts
Immuno-precipitation step
Protein A
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 03 2009

Western blot abreview for Anti-SMC3 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cytosolic and nuclear extracts)
Loading amount
50 µg
Specification
HeLa cytosolic and nuclear extracts
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 02 2009

Immunocytochemistry abreview for Anti-SMC3 antibody - ChIP Grade

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

Submitted Dec 07 2005

Western blot abreview for Anti-SMC3 antibody - ChIP Grade

Good
Abreviews
Abreviews
Application
Western blot
Sample
Xenopus laevis Purified protein (Chromatin isolated from Xenopus egg extract)
Specification
Chromatin isolated from Xenopus egg extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Alan Score

Verified customer

Submitted Nov 28 2005

Question

I would like to use the rabbit polyclonal anti-SMC3 antibody (ChIP-grade), ref ab9263 to detect SMC3 in an invertebrate. The antibody is raised against a peptide derived from the last 100 aa of human SMC3 and this region is well-conserved, but not completely, in the organism that interests me.

Read More

Abcam community

Verified customer

Asked on Mar 12 2013

Answer


I have performed an alignment of the sequence provided in the word document with the sequence used for ab9263. At the site recognized by ab9263, the human and Schistosoma mansoni (blood fluke) sequences are too divergent to expect ab9263 to bind.

Please let me know however if you would like to test ab74582 in Schistosoma mansoni.

Read More

Abcam Scientific Support

Answered on Mar 12 2013

Question

I would like to use the rabbit polyclonal anti-SMC3 antibody (ChIP-grade), ref ab9263 to detect SMC3 in an invertebrate. The antibody is raised against a peptide derived from the last 100 aa of human SMC3 and this region is well-conserved, but not completely, in the organism that interests me. Would it be possible to know the sequence of the peptide that was used to generate the antibody, or failing that, could you analyse the corresponding sequence in my species of interest in order to determine whether or not it corresponds to the peptide?

Read More

Abcam community

Verified customer

Asked on Mar 11 2013

Answer



Unfortunately, at the site recognized by ab9263, the human and Schistosoma mansoni sequences are too divergent to expect ab9263 to bind.

However, Anti-SMC3 antibody's (ab74582) immunogen shows a 90% sequence homology with the above sequence and therefore could work in Schistosoma mansoni.

Read More

Abcam Scientific Support

Answered on Mar 11 2013

Question

I am now informed that it was the following antibody that we were told had been discontinued: ab3600 Rabbit Argonaute 4 antibody Could you please confirm?

Read More

Abcam community

Verified customer

Asked on Jul 02 2004

Answer

Ab3600 - Rabbit polyclonal to Argonaute 4 - has not been discontinued; it is currently in stock in our office in the UK. If you have any more questions, please contact me again.

Read More

Abcam Scientific Support

Answered on Jul 02 2004

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