Recombinant
RabMAb

Recombinant Anti-SMC3 antibody [EPR7984] - BSA and Azide free (ab225553)

Overview

  • Product name

    Anti-SMC3 antibody [EPR7984] - BSA and Azide free
    See all SMC3 primary antibodies
  • Description

    Rabbit monoclonal [EPR7984] to SMC3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C-terminus of Human SMC3 (Q9UQE7).

  • Positive control

    • Human colon tissue. HeLa, A431, K562 and HepG2 cell lysates.
  • General notes

    Ab225553 is the carrier-free version of ab128919. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab225553 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 141 kDa.Can be blocked with SMC3 peptide (ab209493).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
    • Involvement in disease

      Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
    • Sequence similarities

      Belongs to the SMC family. SMC3 subfamily.
    • Domain

      The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
    • Cellular localization

      Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
    • Information by UniProt
    • Database links

    • Alternative names

      • BAM antibody
      • Bamacan antibody
      • Basement membrane associated chondroitin proteoglycan antibody
      • Basement membrane-associated chondroitin proteoglycan antibody
      • BMH antibody
      • CDLS3 antibody
      • chondroitin sulfate proteoglycan 6 (bamacan) antibody
      • Chondroitin sulfate proteoglycan 6 antibody
      • Chromosome associated polypeptide antibody
      • Chromosome-associated polypeptide antibody
      • CSPG 6 antibody
      • CSPG6 antibody
      • hCAP antibody
      • Human chromosome associated polypeptide antibody
      • im:7142991 antibody
      • SMC 3 antibody
      • SMC protein 3 antibody
      • SMC-3 antibody
      • smc3 antibody
      • SMC3_HUMAN antibody
      • SMC3L1 antibody
      • Structural maintenance of chromosome 3 antibody
      • Structural maintenance of chromosomes 3 antibody
      • Structural maintenance of chromosomes protein 3 antibody
      • u:fb22e01 antibody
      • wu:fc30d07 antibody
      see all

    Images

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128919).

    • This IHC data was generated using the same anti-SMC3 antibody clone [EPR7984] in a different buffer formulation (cat# ab128919).

      ab128919 at 1/100 dilution staining SMC3 in paraffin-embedded Human colon tissue by immunohistochemistry.

       

    References

    This product has been referenced in:

    • Yu C  et al. CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation. Nat Commun 6:8017 (2015). ICC/IF ; Mouse . Read more (PubMed: 26281983) »
    See 1 Publication for this product

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