Description

  • Product name

    SMC3 peptide
  • Animal free

    No
  • Nature

    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab209493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-SMC3 antibody [EPR7984] (ab128919)

  • Form

    Liquid
  • Additional notes

    This is the blocking peptide for ab128919

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

General Info

  • Alternative names

    • BAM
    • Bamacan
    • Basement membrane associated chondroitin proteoglycan
    • Basement membrane-associated chondroitin proteoglycan
    • BMH
    • CDLS3
    • Chondroitin sulfate proteoglycan 6
    • chondroitin sulfate proteoglycan 6 (bamacan)
    • Chromosome associated polypeptide
    • Chromosome-associated polypeptide
    • CSPG 6
    • CSPG6
    • hCAP
    • Human chromosome associated polypeptide
    • im:7142991
    • SMC 3
    • SMC protein 3
    • SMC-3
    • smc3
    • SMC3_HUMAN
    • SMC3L1
    • Structural maintenance of chromosome 3
    • Structural maintenance of chromosomes 3
    • Structural maintenance of chromosomes protein 3
    • u:fb22e01
    • wu:fc30d07
    see all
  • Function

    Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
  • Involvement in disease

    Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
  • Sequence similarities

    Belongs to the SMC family. SMC3 subfamily.
  • Domain

    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
  • Information by UniProt

References

ab209493 has not yet been referenced specifically in any publications.

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