Key features and details
- Rabbit polyclonal to SMC3 (phospho S1083)
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SMC3 (phospho S1083) antibody
See all SMC3 primary antibodies
DescriptionRabbit polyclonal to SMC3 (phospho S1083)
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Orangutan
Synthetic peptide within Human SMC3 (phospho S1083). The exact sequence is proprietary. NP_005436.1
Database link: Q9UQE7
- WB: HeLa (treated with ectoposide) whole cell lysate. IP: HeLa (treated with ectoposide) whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 141 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCentral component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
Involvement in diseaseDefects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
Sequence similaritiesBelongs to the SMC family. SMC3 subfamily.
DomainThe flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
- Information by UniProt
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All lanes : Anti-SMC3 (phospho S1083) antibody (ab264351) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) (treated with 100 uM ectoposide for 4 hours) whole cell lysate
Lane 2 : HeLa (treated with 100 uM ectoposide + 100 uM phosphatase for 4 hours) whole cell lysate
Lane 3 : Untreated HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 141 kDa
Upper panel: 3 minutes.
Lower panel: 10 seconds.
SMC3 (phospho S1083) was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264351 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264351 at 1 μg/ml.
Lane 1: ab264351 IP in HeLa (100 uM ectoposide for 4 hours) whole cell lysate.
Lane 2: ab264351 IP in HeLa (untreated) whole cell lysate.
Lane 3: Control IgG IP in Hela (ectoposide for 4 hours) whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264351 has not yet been referenced specifically in any publications.