• Product name
    Anti-SMC3 (phospho S383) antibody
    See all SMC3 primary antibodies
  • Description
    Rabbit polyclonal to SMC3 (phospho S383)
  • Host species
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Rabbits were immunized with a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes-3 (LocusLink ID 9126)around Serine 383, conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected.



Our Abpromise guarantee covers the use of ab1277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 2 - 6 µg/ml.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
    • Involvement in disease
      Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
    • Sequence similarities
      Belongs to the SMC family. SMC3 subfamily.
    • Domain
      The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
    • Cellular localization
      Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
    • Information by UniProt
    • Database links
    • Alternative names
      • BAM antibody
      • Bamacan antibody
      • Basement membrane associated chondroitin proteoglycan antibody
      • Basement membrane-associated chondroitin proteoglycan antibody
      • BMH antibody
      • CDLS3 antibody
      • chondroitin sulfate proteoglycan 6 (bamacan) antibody
      • Chondroitin sulfate proteoglycan 6 antibody
      • Chromosome associated polypeptide antibody
      • Chromosome-associated polypeptide antibody
      • CSPG 6 antibody
      • CSPG6 antibody
      • hCAP antibody
      • Human chromosome associated polypeptide antibody
      • im:7142991 antibody
      • SMC 3 antibody
      • SMC protein 3 antibody
      • SMC-3 antibody
      • smc3 antibody
      • SMC3_HUMAN antibody
      • SMC3L1 antibody
      • Structural maintenance of chromosome 3 antibody
      • Structural maintenance of chromosomes 3 antibody
      • Structural maintenance of chromosomes protein 3 antibody
      • u:fb22e01 antibody
      • wu:fc30d07 antibody
      see all


    • Immunoprecipitation:

      • ab1277 (Rabbit polyclonal to phospho SMC 3 (Ser 383)) at a 2.0µg concentration immunoprecipitating pSMC 3; and ab9263 (Rabbit polyclonal to human SMC 3) at a 2.0µg concentration immunoprecipitating SMC 3, each from 200µg of HeLa nuclear cell extract +/- IR treatment. 

      Western Blot of Immunoprecipitation products and also neat Hela nuclear cell extract +/- IR treatment: 

      • Lanes were visualised using ab9263 at a 1:1000 dilution by Western Blot (ECL).


    This product has been referenced in:
    • Barbeito AG  et al. Abnormal iron metabolism and oxidative stress in mice expressing a mutant form of the ferritin light polypeptide gene. J Neurochem 109:1067-78 (2009). Read more (PubMed: 19519778) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (HeLa)
    Yes - 0.5% Triton X100 in PBS

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Dec 03 2007


    Thank you for contacting us. It seems to me that the phosphorylation referred to on our datasheet for ab1277, anti-phospho SMC3, is of BRCA1: "SMC3 is involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM". I hope this makes sense; if not, please contact us since we value our customer's input. Thank you for bringing this to our attention.

    Read More


    Thank you for your email. Can you please indicate the Abcam order number or purchase order number that was used when you purchased ab1277? Thank you, and I look forward to hearing from you.

    Read More


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