Overview

  • Product name
  • Description
    Rabbit polyclonal to SMC4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ChIP, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide representing aportion of human SMC4 lying within exon 23 (C terminal).

  • Positive control
    • 293T cells, normal human lymphoblasts

Properties

Applications

Our Abpromise guarantee covers the use of ab17958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution. PubMed: 20622854
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 150 kDa).
IP Use at an assay dependent concentration.

Target

  • Function
    Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • Tissue specificity
    Widely expressed. Higher expression in testis, colon, thymus.
  • Sequence similarities
    Belongs to the SMC family. SMC4 subfamily.
  • Domain
    The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.
  • Cellular localization
    Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAP C antibody
    • CAPC antibody
    • Chromosome associated polypeptide C antibody
    • Chromosome-associated polypeptide C antibody
    • hCAP C antibody
    • hCAP-C antibody
    • SMC 4 antibody
    • SMC protein 4 antibody
    • SMC-4 antibody
    • SMC4 antibody
    • SMC4_HUMAN antibody
    • SMC4L1 antibody
    • Structural maintenance of chromosomes 4 antibody
    • Structural maintenance of chromosomes 4 like 1 antibody
    • Structural maintenance of chromosomes protein 4 antibody
    • XCAP C homolog antibody
    • XCAP-C homolog antibody
    see all

Images

  • ab17958 at 0.2µg/ml detecting SMC4 from 90µg of whole cell lysate from 293T cells (left hand lane) or from normal human lymphoblasts (right hand lane) by western blot. The blot was detected by ECL. ab17958 at 0.2µg/ml detecting SMC4 from 90µg of whole cell lysate from 293T cells (left hand lane) or from normal human lymphoblasts (right hand lane) by western blot. The blot was detected by ECL.
  • ICC/IF image of ab17958 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17958, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • SMC4 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal SMC4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17958.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 200kDa: SMC4; non specific - 65kDa: We are unsure as to the identity of this extra band.

References

This product has been referenced in:
  • Xing X  et al. Nesprin-2 Interacts with Condensin Component SMC2. Int J Cell Biol 2017:8607532 (2017). Read more (PubMed: 29445399) »
  • Gerasimova T  et al. A structural hierarchy mediated by multiple nuclear factors establishes IgH locus conformation. Genes Dev 29:1683-95 (2015). ChIP ; Mouse . Read more (PubMed: 26302788) »
See all 8 Publications for this product

Customer reviews and Q&As

Question

Please, could you send me the sequence of the peptide used to raise the anti-SMC4 antibody. Or if you are unable to do that, could you let me know whether the peptide sequence can be found within the SMC4-like protein with NCBI sequence number GI:30173386. Thanks. The sequence of the whole protein is:- 1 mprkgtqpst arrreegppp pspdgassda epeppsgrte spataaetas eeldnrslee 61 ilnsippppp pamtneagap rlmithivnq nfksyageki lgpfhkrfsc iigpngsgks 121 nvidsmlfvf gyraqkirsk klsvlihnsd ehkdiqsctv evhfqkiidk egddyevipn 181 snfyvsrtac rdntsvyhis gkkktfkdvg nllrshgidl dhnrflilqg eveqiammkp 241 kgqtehdegm leylediigc grlnepikvl crrveilneh rgeklnrvkm vekekdaleg 301 ekniaieflt leneifrkkn hvcqyyiyel qkriaemetq kekihedtke ineksnilsn 361 emkaknkdvk dtekklnkit kfieenkekf tqldledvqv reklkhatsk akklekqlqk 421 dkekveefks ipaksnniin etttrnnale kekekeekkl kevmdslkqe tqglqkekes 481 rekelmgfsk svnearskmd vaqseldiyl srhntavsql tkakealiaa setlkerkaa 541 irdiegklpq teqelkekek elqkltqeet nfkslvhdlf qkveeakssl amnrsrgkvl 601 daiiqekksg ripgiygrlg dlgaidekyd vaisscchal dyivvdsidi aqecvnflkr 661 qnigvatfig ldkmavwakk mteiqtpent prlfdlvkvk dekirqafyf alrdtlvadn 721 ldqatrvayq kdrrwrvvtl qgqiieqsgt mtgggskvmk grmgsslvie iseeevnkme 781 sqlqndskka mqiqeqkvql eervvklrhs eremrntlek ftasiqrlie qeeylnvqvk 841 eleanvlata pdkkkqklle envsafktey davaekagkv eaevkrlhnt iveinnhklk 901 aqqdkldkin kqldecasai tkaqvaikta drnlqkaqds vlrtekeikd tekevddlta 961 elksledkaa evvkntnaae eslpeiqkeh rnllqelkvi qenehalqkd alsiklkleq 1021 idghiaehns kikywhkeis kislhpiedn pieeisvlsp edleaiknpd sitnqialle 1081 archemkpnl gaiaeykkke elylqrvael dkityerdsf rqayedlrkq rlnefmagfy 1141 iitnklkeny qmltlggdae lelvdsldpf segimfsvrp pkkswkkifn lsggektlss 1201 lalvfalhhy kptplyfmde idaaldfknv sivafyiyeq tknaqfiiis lrnnmfeisd 1261 rligiyktyn itksvavnpk eiaskglc

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Answer

Thank you for your enquiry. I can confirm that the peptide is conserved in your sequence (GI:30172286). The reference used in selecting peptide antigens for human SMC4 was Swiss Prot entry Q9NTJ3 (the link to this entry can be found on the product data sheet).It would appear that SMC4, SMC4L1 and CAPC are synonyms for the same gene product (Gene ID:10051). If you have nay further questions then please get back in touch with us.

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