Key features and details
- Rabbit polyclonal to SMC4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.2% Gelatin, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab78973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 147 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionCentral component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Tissue specificityWidely expressed. Higher expression in testis, colon, thymus.
Sequence similaritiesBelongs to the SMC family. SMC4 subfamily.
DomainThe hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.
Cellular localizationNucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
- Information by UniProt
- CAP C antibody
- CAPC antibody
- Chromosome associated polypeptide C antibody
ab78973 has not yet been referenced specifically in any publications.