Overview

  • Product name

    Anti-SMC4 antibody - C-terminal
    See all SMC4 primary antibodies
  • Description

    Rabbit polyclonal to SMC4 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human SMC4 (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
    Database link: Q9NTJ3

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab229480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 147 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • Tissue specificity

    Widely expressed. Higher expression in testis, colon, thymus.
  • Sequence similarities

    Belongs to the SMC family. SMC4 subfamily.
  • Domain

    The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.
  • Cellular localization

    Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAP C antibody
    • CAPC antibody
    • Chromosome associated polypeptide C antibody
    • Chromosome-associated polypeptide C antibody
    • hCAP C antibody
    • hCAP-C antibody
    • SMC 4 antibody
    • SMC protein 4 antibody
    • SMC-4 antibody
    • SMC4 antibody
    • SMC4_HUMAN antibody
    • SMC4L1 antibody
    • Structural maintenance of chromosomes 4 antibody
    • Structural maintenance of chromosomes 4 like 1 antibody
    • Structural maintenance of chromosomes protein 4 antibody
    • XCAP C homolog antibody
    • XCAP-C homolog antibody
    see all

Images

  • All lanes : Anti-SMC4 antibody - C-terminal (ab229480) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 147 kDa



    5% SDS-PAGE

  • SMC4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg ab229480. Western blot was performed from the immunoprecipitate using ab229480.

    Lane 1: HEK-293T whole cell extract (Input)

    Lane 2: Control IgG IP in HEK-293T whole cell extract.

    Lane 3: ab229480 IP in HEK-293T whole cell extract.

References

ab229480 has not yet been referenced specifically in any publications.

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