Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13366] to SMC4 - C-terminal
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
Product nameAnti-SMC4 antibody [EPR13366] - C-terminal
See all SMC4 primary antibodies
DescriptionRabbit monoclonal [EPR13366] to SMC4 - C-terminal
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SMC4 aa 1200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NTJ3
- Ramos, 293T, HeLa and Raji cell lysates. HeLa and Raji cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab179831 in the following tested applications.
|WB||1/1000 - 1/10000. Predicted molecular weight: 147 kDa.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCentral component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Tissue specificityWidely expressed. Higher expression in testis, colon, thymus.
Sequence similaritiesBelongs to the SMC family. SMC4 subfamily.
DomainThe hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.
Cellular localizationNucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
- Information by UniProt
- CAP C antibody
- CAPC antibody
- Chromosome associated polypeptide C antibody
All lanes : Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 147 kDa
Immunofluorescent analysis of HeLa cells labeling SMC4 with ab179831 at 1/50 dilution.
Flow cytometric analysis of permeabilized Raji cells using ab179831 at 1/10 (red) or a rabbit IgG negative (green).
ab179831 has been referenced in 2 publications.
- Baergen AK et al. Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis. Cancers (Basel) 11:N/A (2019). PubMed: 31357676
- Meng Q et al. The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence. Biochem Biophys Res Commun 505:1112-1120 (2018). PubMed: 30336977