Overview

  • Product name

    Anti-SMC4 antibody [EPR13366] - C-terminal
    See all SMC4 primary antibodies
  • Description

    Rabbit monoclonal [EPR13366] to SMC4 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SMC4 aa 1200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NTJ3

  • Positive control

    • Ramos, 293T, HeLa and Raji cell lysates. HeLa and Raji cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 147 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
    • Tissue specificity

      Widely expressed. Higher expression in testis, colon, thymus.
    • Sequence similarities

      Belongs to the SMC family. SMC4 subfamily.
    • Domain

      The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.
    • Cellular localization

      Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
    • Information by UniProt
    • Database links

    • Alternative names

      • CAP C antibody
      • CAPC antibody
      • Chromosome associated polypeptide C antibody
      • Chromosome-associated polypeptide C antibody
      • hCAP C antibody
      • hCAP-C antibody
      • SMC 4 antibody
      • SMC protein 4 antibody
      • SMC-4 antibody
      • SMC4 antibody
      • SMC4_HUMAN antibody
      • SMC4L1 antibody
      • Structural maintenance of chromosomes 4 antibody
      • Structural maintenance of chromosomes 4 like 1 antibody
      • Structural maintenance of chromosomes protein 4 antibody
      • XCAP C homolog antibody
      • XCAP-C homolog antibody
      see all

    Images

    • All lanes : Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 147 kDa

    • Immunofluorescent analysis of HeLa cells labeling SMC4 with ab179831 at 1/50 dilution.

    • Flow cytometric analysis of permeabilized Raji cells using ab179831 at 1/10 (red) or a rabbit IgG negative (green).

    References

    This product has been referenced in:

    • Meng Q  et al. The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence. Biochem Biophys Res Commun 505:1112-1120 (2018). Read more (PubMed: 30336977) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab179831.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up