Product nameAnti-SMC4 antibody - N-terminal
See all SMC4 primary antibodies
DescriptionRabbit polyclonal to SMC4 - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human SMC4 (N terminal). The exact sequence is proprietary.
Database link: Q9NTJ3
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 147 kDa.|
|IP||1/100 - 1/500.|
FunctionCentral component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Tissue specificityWidely expressed. Higher expression in testis, colon, thymus.
Sequence similaritiesBelongs to the SMC family. SMC4 subfamily.
DomainThe hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.
Cellular localizationNucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
- Information by UniProt
- CAP C antibody
- CAPC antibody
- Chromosome associated polypeptide C antibody
All lanes : Anti-SMC4 antibody - N-terminal (ab229213) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 147 kDa
5% SDS-PAGE gel.
SMC4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229213. Western blot was performed from the immunoprecipitate using ab229213. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG instead of ab229213 in HEK-293T whole cell extract.
Lane 3: ab229213 IP in HEK-293T whole cell extract.
ab229213 has not yet been referenced specifically in any publications.