The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/25000. Detects a band of approximately 145 kDa (predicted molecular weight: 129 kDa).
Use a concentration of 5 - 10 µg/ml.
Is unsuitable for ICC/IF.
Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics.
Nucleus. Chromosome. Chromosome; telomere. Note: Associates with chromatin. Localizes to PML nuclear bodies in ALT cell lines.
Gallego-Paez LM et al. Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells. Mol Biol Cell25:302-17 (2014).
Read more (PubMed: 24258023) »
Gómez R et al. Dynamic localization of SMC5/6 complex proteins during mammalian meiosis and mitosis suggests functions in distinct chromosome processes. J Cell Sci126:4239-52 (2013).
Read more (PubMed: 23843628) »